ANALYSIS OF THE ANAEROBIC-STRESS RESPONSE IN MAIZE

玉米厌氧胁迫反应的分析

• 批准号: 3286244
• 负责人: MARTIN M SACHS
• 金额: $ 17.01万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-04-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3286244
• 关键词: alcohol dehydrogenase; anaerobiosis; corn; developmental genetics; disasters; environmental stressor; gene expression; genetic library; genetic manipulation; genetic mapping; genetic transcription; genetic translation; immunochemistry; isozymes; laboratory mouse; messenger RNA; molecular cloning; nucleic acid sequence; plant genetics; plant proteins; protein biosynthesis; regulatory gene; seeds; stress; stress proteins

I propose to study the anaerobic-stress response in Zea mays. Anaerobic treatment dramatically alters the pattern of gene expression in maize seedlings. During anaerobiosis there is an immediate repression of pre-existing protein synthesis followed by an induction of gene expression involving the synthesis of approximately twenty anaerobic-specific proteins. Among these anaerobic proteins (ANPs) are the alcohol dehydrogenase (ADH) isozymes encoded by the Adhl and Adh2 genes. The anaerobic-stress response of maize offers a unique opportunity to characterize the regulatory components of a family of twenty genes which are coordinately expressed. The overall aim of this proposal is to use the powerful tools of genetics and recombinant DNA technology to analyze this highly regulated response. I plan to identify and characterize the genes involved in a plant's response to an environmental stress which would occur in nature during flooding. These genes include the remaining anaerobic- specific genes, and those encoding the transition polypeptides (TPs), which are a group of protein synthesized preferentially during the first few hours of anaerobic treatment. It also includes possible trans-acting genes that might control this physiological response. Sequence comparisons and expression studies will be carried out to identify regions within the anaerobic genes that may be responsible for the induction of transcription and the selective translation of their mRNAs during anaerobiosis. It is anticipated that these studies will contribute toward the understanding of the eukaryotic gene as a functional unit and to some aspects of differential gene expression.

我建议研究玉米的厌氧应激反应。 厌氧处理显著改变了基因的模式， 在玉米幼苗中的表达。 在厌氧期间， 立即抑制预先存在的蛋白质合成， 诱导基因表达，包括合成 大约20种厌氧特异性蛋白质。 其中 厌氧蛋白（ANP）是乙醇脱氢酶（ADH） 由Adh 1和Adh 2基因编码的同工酶。 玉米的厌氧应激反应提供了一个独特的机会 来描述一个20人家庭的调节成分， 协调表达的基因。 其总体目标是 建议是使用强大的遗传学和重组工具， DNA技术来分析这种高度调节的反应。 我计划 来识别和描述植物基因的特征， 自然界中对环境压力的反应 在洪水期间。 这些基因包括剩余的厌氧- 特异性基因和编码过渡多肽的基因 (TPs)，它们是一组优先合成的蛋白质 在厌氧处理的最初几个小时内。 它还 包括可能的反式作用基因， 生理反应。 序列比较和表达 将进行研究，以确定 厌氧基因，可能是负责诱导 转录及其mRNA的选择性翻译， 厌氧生活 预计这些研究将有助于 了解真核基因作为一个功能单位， 差异基因表达的一些方面。