ANALYSIS OF THE ANAEROBIC-STRESS RESPONSE IN MAIZE

我建议研究玉米的厌氧胁迫反应。 厌氧处理显著改变了基因的模式 在玉米幼苗中的表达。在厌氧期间，有一种 立即抑制先前存在的蛋白质合成，然后 对基因表达的诱导，涉及合成 大约20种厌氧特有的蛋白质。其中包括 厌氧蛋白(ANP)是酒精脱氢酶(ADH) Adh1和adh2基因编码的同工酶。 玉米的厌氧胁迫反应提供了一个独特的机会 描述一个二十口之家的监管成分 协调表达的基因。这样做的总体目标是 建议是利用遗传学和重组的强大工具 DNA技术来分析这种高度调控的反应。我计划 识别和表征植物中涉及的基因 对自然界中可能发生的环境压力的反应 在洪水期间。这些基因包括剩余的厌氧菌- 特定基因，以及那些编码过渡多肽的基因 (TPS)，这是一组优先合成的蛋白质 在厌氧处理的最初几个小时内。它还 包括可能控制这一现象的反式作用基因 生理反应。序列比较和表达 将进行研究，以确定 厌氧基因，可能是导致 它们的转录和选择性翻译 厌氧症。 预计这些研究将有助于 对真核基因作为一个功能单位的理解 差异基因表达的某些方面。

I propose to study the anaerobic-stress response in Zea mays. Anaerobic treatment dramatically alters the pattern of gene expression in maize seedlings. During anaerobiosis there is an immediate repression of pre-existing protein synthesis followed by an induction of gene expression involving the synthesis of approximately twenty anaerobic-specific proteins. Among these anaerobic proteins (ANPs) are the alcohol dehydrogenase (ADH) isozymes encoded by the Adhl and Adh2 genes. The anaerobic-stress response of maize offers a unique opportunity to characterize the regulatory components of a family of twenty genes which are coordinately expressed. The overall aim of this proposal is to use the powerful tools of genetics and recombinant DNA technology to analyze this highly regulated response. I plan to identify and characterize the genes involved in a plant's response to an environmental stress which would occur in nature during flooding. These genes include the remaining anaerobic- specific genes, and those encoding the transition polypeptides (TPs), which are a group of protein synthesized preferentially during the first few hours of anaerobic treatment. It also includes possible trans-acting genes that might control this physiological response. Sequence comparisons and expression studies will be carried out to identify regions within the anaerobic genes that may be responsible for the induction of transcription and the selective translation of their mRNAs during anaerobiosis. It is anticipated that these studies will contribute toward the understanding of the eukaryotic gene as a functional unit and to some aspects of differential gene expression.