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Bactericidal principle of the fermented aqueous extract of Stevia rebaudiana Bertoni and a possible use of the Stevia extract as a feed supplement for prevention of the digestive tract infections

甜叶菊发酵水提取物的杀菌原理以及甜叶菊提取物作为预防消化道感染的饲料补充剂的可能用途

基本信息

批准号：
10556014
负责人：
KAMIO Yoshiyuki
金额：
$ 8.58万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

We have previously shown that a fermenteds extract from the Paraguayan composite plant, Stevia rebaudiana Bertoni has strong bactericidal activity towards a wide range of food-borne pathogenic bacteria, but it shows no significant killing activity to Bifidobacteria and Lactobacilli. Here we studied bactericidal principle of the fermented extract, and tested the extract for possible use as a feed supplement for prevention of the intestinal tract infections of domestic animals.1. Fermented aqueous extract of Stevia rebaudiana Bertoni was fractionated by the partition with ethyl acetate, followed by the high performance column chromatography using an ODS (CィイD218ィエD2) column. Two bactericidal fractions and one enhancer fraction were obtained. Viability of E. coli was reduced from 10ィイD19ィエD1 cfu/ml to <10ィイD13ィエD1 cfu/ml when incubated with the bactericidal fractions at 37℃ for 1h. One bactericidal fraction has he maximal absorption at 280 nm, and it was sensitive to light irradiation. The other bactericidal fraction was identified as lactic acid.2. In the laboratory experiments, oral administration of the Stevia extract to the Listeria-infected mice decreased the numbers of viable bacteria in the intestinal tract from 10ィイD17ィエD1 cfu/ml to 10ィイD13ィエD1 cfu/ml within 3 days. Oral administration of the extract to the Salmonella-infected calves reduced viable bacteria in the intestinal tract and bacterial tissue invasion.3. In the field experiments, oral administration of the Stevia extract was effective to the intestinal tract infections of piggys. Many of the animals with early-staged diarrhea were recovered within 3 days after the treatment, and their growth rate was normal. Oral administration of the diluted Stevia extract promoted growth of pigs, and the quality of the pork meat was evaluated high at the slaughter house.

我们以前已经证明，从菊科植物甜叶菊Bertoni的发酵提取物对广泛的食源性致病菌具有很强的杀菌活性，但它对双歧杆菌和乳酸杆菌没有显示出显著的杀灭活性。在这里，我们研究了发酵提取物的杀菌原理，并测试了提取物作为饲料补充剂预防家畜肠道感染的可能性。1.甜菊发酵后的水提物经乙酸乙酯萃取后，用ODS（C12 H218）柱进行高效柱层析分离。得到两个杀菌级分和一个增强剂级分。E.大肠杆菌在37℃培养1h后，杀菌活性由10 μ g/L D19 μ g/L D1cfu/ml降至<10 μ g/L D13 μ g/L D1cfu/ml。其中一个杀菌组分在280 nm处有最大吸收，对光敏感。另一个杀菌组分被鉴定为乳酸.在实验室实验中，对李斯特氏菌感染的小鼠口服甜叶菊提取物，在3天内将肠道中的活菌数量从10 cfu/ml D17 cfu/ml D1降至10 cfu/ml D13 cfu/ml D1。对沙门氏菌感染的小牛口服提取物减少了肠道中的活菌和细菌组织侵入。在田间试验中，甜菊提取物口服给药对猪肠道感染有效。许多早期腹泻的动物在治疗后3天内恢复，其生长速度正常。口服给药稀释的甜叶菊提取物促进了猪的生长，并且在屠宰场对猪肉的质量进行了高评价。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Toshio TOMITA, Naohiko SATO, Minoru SATO, Hiroyuki SHIRAISHI, Masayuki TAKEUCHI, and Yoshiyuki KAMIO: "Bactericidal activity of a fermented extract of Stevia rebaudiana Bertoni towards enterohaemorrhagic Escherichiia colli and other food-borne pathogenic

Toshio TOMITA、Naohiko SATO、Minoru SATO、Hiroyuki Shiraishi、Masayuki TAKEUCHI 和 Yoshiyuki KAMIO：“甜叶菊发酵提取物对肠出血性大肠杆菌和其他食源性致病菌的杀菌活性

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0
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通讯作者：

富田敏夫、神尾好是、佐藤直彦: "食中毒菌に対する選択的殺菌剤"公開特許広報特開平11-43443. (1999)

Toshio Tomita、Yoshiko Kamio、Naohiko Sato：“针对食物中毒细菌的选择性消毒剂”专利公开 JP-A-11-43443 (1999)。

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0
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冨田敏夫、佐藤直彦、佐藤*、荒井富雄、白石廣行、神尾好是: "腸管出血性大腸菌O157:H7及び他の食中毒起因菌に対するステビア発酵液の殺菌効果"日本農芸化学会誌(大会講演要旨集). 72. 126 (1998)

Toshio Tomita、Naohiko Sato、Sato*、Tomio Arai、Hiroyuki Shiraishi、Yoshizuru Kamio：“甜叶菊发酵液对肠出血性大肠杆菌 O157:H7 和其他引起食物中毒的细菌的杀菌作用”日本农业化学学会杂志（会议）摘要）) 72. 126 (1998)。