Prevention of Alzheimer disease by oral bacteria having plasmalogenphospholipid

我们在人类口腔细菌Selenomonas sputigena中发现了纤溶酶原磷脂(Pls)，它被认为可以预防阿尔茨海默病(AD)。已知阿尔茨海默病患者脑内PERS减少。淀粉分泌酶(γγS)是已知的裂解淀粉样前体蛋白生成淀粉样多肽(Aββ)，沉积在阿尔茨海默病患者的大脑中。在这里，我们利用福泰等人建立的酿酒酵母体外检测系统，研究了人脑中磷脂组成与γ-分泌酶产生的关系。结果表明：(1)添加磷脂酰胆碱(PC)可促进β的产生；(2)在体外测定体系中加入磷脂酰乙醇胺(EPPLs)可抑制γ-S的活性。因此，我们得出结论，人脑磷脂部分EPPls的减少增强了γS的活性，并导致AD患者Aβ的产生和/或蓄积。这在世界上尚属首次报道。

We found plasmalogen phospholipids (Pls), which are known to preventeAlzheimer disease (AD), in a human oral bacteria, Selenomonas sputigena. It has been known that Pls decrease in brain of AD patient. γ-Secretase(γS) is known to cleave amyloid precursor protein toproduce amyloid β peptides (Aβ) that is deposited in the brain of Alzheimer disease. Here we examined a relationship between phospholipid composition and production of γ-secretase in human brain, by in vitro assay system using Saccharomyces cerevisiae, which was established by Futai et al. The followings are evident, (1) Aβ-production is enhanced by the addition of phosphatidyl choline (PC), and (2) addition of phosphatidyl ethanolamine Pls (EPPls) to in vitro assay system prevented γS activity. Thus we concluded that the reduction of EPPls in the phospholipid fraction in human brain enhances the γS activity and causes Aβ-production and/or accumulation in AD patient. This is a first report in theworld.