Retention slectivity achiebed by the stationary phase modified with mucoplysaccharides in liquid chromatography

本文研究了离子色谱中被分析物离子在粘多糖和硫酸葡聚糖等阴离子多糖修饰的硅基阴离子交换剂上的保留行为。改性后基质的阴离子交换容量显著降低，而阳离子交换性能得到诱导。改性的固定相具有残留的阴离子交换位点的基板以及阳离子交换位点，由于自由的阴离子基团的改性剂。基质的孔径和改性剂的分子量强烈影响分析物离子的保留行为。由于阴离子和阳离子都保留在改性固定相上，洗脱液的阳离子和阴离子影响分析物离子的保留行为。在适当的条件下，阴离子和阳离子在改性固定相上可同时分离，用肝素改性阴离子交换树脂作固定相，对氯喹和其他胺类衍生物的对映体进行了液相色谱分离。修饰和移动的条件影响分析物的分离。采用乙腈缓冲溶液作为移动的流动相，可以实现氯喹对映体的分离。

Retention behavior of analyte ions on silica-based anion exchangers modified with anionic polysaccharides such as mucopolysaccharides and dextran sulfate has been investisated in ion chromatography. The anion-exchange capacity of the substrate was remarkably reduced by the modification, whereas cation-exchange properties were induced. The modified stationary phases possess residual anion-exchange sites of the substrate as well as cation-exchange sites due to free anionic groups of the modifier. The pore size of the substrate and the molecular weight of the modifier strongly affected the retention behavior of analyte ions. Since both anions and cations were retained on the modified stationary phase, the cation and the anion of the eluent affected the retention behavior of analyte ions. Under appropriate conditions both anions and cations were simultaneously separated on the modified stationary phase.Enantiomeric separation of chloroquine and other amine derivatives has been investigated by liquid chromatography using anion-exchangers modifiled with heparin as the stationary phase. Modification and mobile phase conditions affected the separation of analytes. Separation of chloroquine enantiomers could be achieved by using buffer solution containing acetonitrile as the mobile phase.