Retention slectivity achiebed by the stationary phase modified with mucoplysaccharides in liquid chromatography

粘多糖修饰固定相在液相色谱中实现保留选择性

• 批准号: 10640589
• 负责人: TAKEUCHI Toyohide
• 金额: $ 2.11万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10640589/
• 关键词: liquid chromatography; ion chromatography; stationary phases; mucopolysaccharides; retention michanism; separation of anions and cations; enantiomers; 溶離条件; 保持挙動; 無機陽イオン; 分離条件; 陰陽両イオン

Retention behavior of analyte ions on silica-based anion exchangers modified with anionic polysaccharides such as mucopolysaccharides and dextran sulfate has been investisated in ion chromatography. The anion-exchange capacity of the substrate was remarkably reduced by the modification, whereas cation-exchange properties were induced. The modified stationary phases possess residual anion-exchange sites of the substrate as well as cation-exchange sites due to free anionic groups of the modifier. The pore size of the substrate and the molecular weight of the modifier strongly affected the retention behavior of analyte ions. Since both anions and cations were retained on the modified stationary phase, the cation and the anion of the eluent affected the retention behavior of analyte ions. Under appropriate conditions both anions and cations were simultaneously separated on the modified stationary phase.Enantiomeric separation of chloroquine and other amine derivatives has been investigated by liquid chromatography using anion-exchangers modifiled with heparin as the stationary phase. Modification and mobile phase conditions affected the separation of analytes. Separation of chloroquine enantiomers could be achieved by using buffer solution containing acetonitrile as the mobile phase.

离子色谱法研究了分析物离子在用阴离子多糖（例如粘多糖和硫酸葡聚糖）改性的二氧化硅基阴离子交换剂上的保留行为。通过修饰，基质的阴离子交换能力显着降低，而阳离子交换性能却被诱导。改性固定相具有底物的残余阴离子交换位点以及由于改性剂的游离阴离子基团而具有的阳离子交换位点。基质的孔径和改性剂的分子量强烈影响分析物离子的保留行为。由于阴离子和阳离子均保留在改性固定相上，因此洗脱液的阳离子和阴离子会影响分析物离子的保留行为。在适当的条件下，阴离子和阳离子在修饰的固定相上同时分离。采用肝素修饰的阴离子交换剂作为固定相，通过液相色谱法研究了氯喹和其他胺衍生物的对映体分离。修饰和流动相条件影响分析物的分离。使用含有乙腈的缓冲溶液作为流动相可以实现氯喹对映体的分离。

项目成果

T.Takeuchi, Safni, T.Miwa, Y.Hashimoto, H.Moriyama: "Ion chromatography using anion exchangers modified with dextran sulfate" Chromatographia. (印刷中). (1999)

T.Takeuchi、Safni、T.Miwa、Y.Hashimoto、H.Moriyama：“使用用硫酸葡聚糖修饰的阴离子交换剂的离子色谱法”（印刷中）。

Safni, T.Takeuchi, T.Miwa, Y.Hashimoto, H.Moriyama: "Effect of eluent composition on retenti on behavior of anions in ion chromatography on anion exchangers modified with heparin" J.Chromatogr.A. (印刷中). (1999)

Safni、T.Takeuchi、T.Miwa、Y.Hashimoto、H.Moriyama：“洗脱液成分对肝素修饰阴离子交换剂离子色谱中阴离子保留行为的影响”J.Chromatogr.A（出版中）。 (1999)

Safni, T.Takeuchi, T.Miwa, Y.Hashimoto, H.Moriyama: "Effect of eluent composition on retention behavior of anions in ion chromatography on anion exchangers modified with heparin"J.Chromatogr.A. 850. 65-72 (1999)

Safni、T.Takeuchi、T.Miwa、Y.Hashimoto、H.Moriyama：“洗脱液组成对肝素修饰阴离子交换剂离子色谱中阴离子保留行为的影响”J.Chromatogr.A。

T.Takeuchi, Safni, T.Miwa: "Ion chromatography using anion exchangers modified with anionic polysaccharides"LC-GC. 18. 418-428 (2000)

T.Takeuchi、Safni、T.Miwa：“使用用阴离子多糖修饰的阴离子交换剂的离子色谱法”LC-GC。

Safni, N.Ito, T.Takeuchi, T.Miwa,: "Separation of enantiomers on anion exchangers modified with heparin in liquid chromatography"J.Chromatogr.A. 864. 25-30 (1999)

Safni，N.Ito，T.Takeuchi，T.Miwa，：“液相色谱中肝素修饰的阴离子交换剂上对映异构体的分离”J.Chromatogr.A.