High resolution mass spectrometer (HRMS) alternatively coupled to ion chromatography (IC) or liquid chromatography (LC)

用于代谢物分析的高分辨率质谱仪（HRMS）将用于不同的研究领域。该机器可与液相色谱（LC）或离子色谱（IC）耦合。与IC的耦合将用于分析核苷酸和有机酸等带电化合物。质谱仪的一个用途是通过对遗传变异的（亚）代谢组谱进行有针对性的比较，提供核苷酸和氨基酸代谢中基因/蛋白质功能的指示。该机器还将用于阐明生物体内以及环境样品中的代谢通量。此外，（有意引入的）遗传改变对代谢物谱的影响将被研究。样品材料将来自不同的植物（如拟南芥、水稻、花生）以及环境（如沼气植物、蚯蚓粪便）。HRMS应能够可靠地识别和定量这些复杂样品中的低浓度代谢物。

A high resolution mass spectrometer (HRMS) for metabolite analyses will be employed in different research areas. The machine can alternatively be coupled to a liquid chromatography (LC) or an ion chromatography (IC). The coupling to an IC will be used for the analysis of charged compounds like nucleotides and organic acids. One use of the mass spectrometer will be to provide indications about gene / protein functions in nucleotide and amino acid metabolism by performing targeted comparisons of (sub-)metabolome profiles in genetic variants. The machine will also be used to elucidate metabolic fluxes within organisms as well as in environmental samples. Moreover, the consequences of (intentionally introduced) genetic alterations on metabolite profiles will be investigated. Sample material will be derived from different plants (e.g. Arabidopsis, rice, peanut) as well as from the environment (e.g. biogas plant, earthworm excrements). The HRMS shall be able to reliably identify and quantify even low concentrated metabolites in these complex samples.