High resolution mass spectrometer (HRMS) alternatively coupled to ion chromatography (IC) or liquid chromatography (LC)

高分辨率质谱仪 (HRMS) 或者与离子色谱仪 (IC) 或液相色谱仪 (LC) 联用

• 批准号: 423879281
• 金额: --
• 依托单位: Gottfried Wilhelm Leibniz Universität Hannover
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/423879281?language=en
• 关键词: resolution; mass; spectrometer; HRMS; alternatively

A high resolution mass spectrometer (HRMS) for metabolite analyses will be employed in different research areas. The machine can alternatively be coupled to a liquid chromatography (LC) or an ion chromatography (IC). The coupling to an IC will be used for the analysis of charged compounds like nucleotides and organic acids. One use of the mass spectrometer will be to provide indications about gene / protein functions in nucleotide and amino acid metabolism by performing targeted comparisons of (sub-)metabolome profiles in genetic variants. The machine will also be used to elucidate metabolic fluxes within organisms as well as in environmental samples. Moreover, the consequences of (intentionally introduced) genetic alterations on metabolite profiles will be investigated. Sample material will be derived from different plants (e.g. Arabidopsis, rice, peanut) as well as from the environment (e.g. biogas plant, earthworm excrements). The HRMS shall be able to reliably identify and quantify even low concentrated metabolites in these complex samples.

高分辨率质谱仪（HRMS）的代谢物分析将在不同的研究领域。所述机器可替代地耦合到液相色谱（LC）或离子色谱（IC）。与IC的耦合将用于分析带电化合物，如核苷酸和有机酸。质谱仪的一个用途是通过对遗传变异体中的（亚）代谢组谱进行靶向比较，提供有关核苷酸和氨基酸代谢中基因/蛋白质功能的指示。该机器还将用于阐明生物体内以及环境样品中的代谢通量。此外，还将研究（有意引入的）遗传改变对代谢产物谱的影响。样品材料将来自不同的植物（例如拟南芥、水稻、花生）以及环境（例如沼气厂、粪便）。HRMS应能够可靠地识别和定量这些复杂样本中的低浓度代谢物。