A Comparative Study on Aging Processes between Human and Nonhuman Primates based on Biological Age.

本研究的目的是：1）鉴定猴和人衰老的共同生物标志物，比较猴和人的衰老速率; 2）揭示猴衰老过程的基本规律，并应用于人类; 3）研究限制热量摄入是否能延缓灵长类动物的衰老进程（19只年轻成年猴和14只老年猴）。一半的受试者被分配到实验组，与对照组相比，实验组受到30%的热量限制10年。人类受试者为122名日本成年男性，他们从1992年至1998年在京都红十字医院接受了为期7年的2天健康检查。从血液学和血生化中筛选出27个检测指标用于筛选猴衰老的候选生物标志物，结果表明：1）猴血液学中HGB和LYMPH以及血生化中GOT、ALK PHOS、T-PRO、ALBU、AGR、CALC和DHEA等9个指标可作为猴衰老的候选生物标志物; 2）在这9个生物标志物中，HGB、LYMPH、ALBU和CALC 4个变量被鉴定为能够用作人类衰老的生物标志物; 3）猴子的衰老个体差异相对小于人类。猴子和人类之间的这种差异可能反映了寿命的差异。在猴子中，生理功能以线性方式下降可能代表生物体保持稳态的能力下降; 4）我们使用衰老的生物学指数（BAS）研究了CR对衰老速率的影响。结果表明，CR并不像在啮齿动物中报道的那样显著延长寿命。寿命似乎会适度增加; 5）猴子的衰老速度比人类快3.46倍。

The purposes of this study were 1) to identify common biomarkers of aging between monkeys and humans to compare their aging rates ; 2) to reveal basic regularities of the aging process in monkeys which could apply to human ; and 3) to determine if caloric restriction retards the aging processes in primates as it does in rodents.The monkeys used in the present study were 33 male rhesus monkeys (19 young adult and 14 old monkeys). Half of subjects were assigned to experimental group subjected to 30% calorie restriction for 10 years compared to the control group. Subjects for humans were 122 Japanese adult men, who received a 2-day health examination at the Kyoto Red Cross Hospital for 7 years from 1992 to 1998. The 27 test variables selected from hematology and blood chemistry were used to find the candidate biomarkers of aging in monkeys.The results indicated that : 1) In monkeys, 9 variables of HGB and LYMPH from hematology and GOT, ALK PHOS, T-PRO, ALBU, AGR, CALC and DHEA from blood chemisty were selected as possible candidate biomarkers of aging ; 2) Of these 9 biomarkers, 4 variables of HGB, LYMPH, ALBU and CALC were identified to be able to use as biomarkers of aging in humans ; 3) Individual differences of aging are relatively less in monkeys than in humans. This difference between monkeys and humans might reflect the difference of longevity. In monkeys, the decline of physiological functions in a linear fashion may represent a decline in the ability of the organism to preserve homeostasis ; 4) we investigated the influence of CR on the rate of aging, using the biological index (BAS) of aging. The result indicates that the CR does not markedly extend life span as reported in rodents. It seems likely that moderate increases in life span will occur ; and 5) The rate of aging is faster by 3.46 times in monkeys than in humans.