Confocal Microscope for Three-Dimensional Polarization Analysis and Its applications to biology

三维偏振分析共焦显微镜及其在生物学中的应用

• 批准号: 10650041
• 负责人: KAWATA Yoshimasa
• 金额: $ 2.3万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650041/
• 关键词: microscopy; confocal microscopy; polarization microscopy; laser; polarization anlaysis; sigle molecular; biology; optical elasticity

A confocal microscope for the use of three-dimensional polarization analysis was developed. Since the pinhole before the detector eliminates the light whose polarization is rotated by an imaging lens, the polarization properties of specimens are observed with high contrast. The pinhole size required to achieve high contrast was discussed. Three-dimensional resolution of the polarization microscope was also demonstrated.The three-dimensional imaging analysis using the extended Mie scattering theory was developed. In the analysis, we calculated the images of a Mie particle whose diameter is comparable with the wavelength of confocal microscope. It was found that, when we observe a Mie particle, polarization confocal microscopy is not affected by the polarization distortion that is due to focusing with high-numerical-aperture lenses and does not produce pseudopeaks in the images in comparison with conventional polarization microscopy. The three-dimensional resolution of the polarization microscope and the verification of the proposed analysis method were also discussed.

开发了一种用于三维偏振分析的共焦显微镜。由于检测器前面的针孔消除了通过成像镜头旋转偏振的光，因此可以以高对比度观察样本的偏振特性。讨论了实现高对比度所需的针孔尺寸。还展示了偏振显微镜的三维分辨率。开发了使用扩展米氏散射理论的三维成像分析。在分析中，我们计算了直径与共焦显微镜波长相当的米氏颗粒的图像。研究发现，当我们观察米氏粒子时，与传统的偏光显微镜相比，偏光共焦显微镜不受高数值孔径透镜聚焦引起的偏光畸变的影响，并且不会在图像中产生伪峰。还讨论了偏光显微镜的三维分辨率以及所提出的分析方法的验证。

项目成果

Y.Kawata: "Three-dimensional imaging analysis of confocal polarization microscopy using Mie scattering theroy"Appl.Opt.. 39. 6369-6373 (2000)

Y.Kawata：“使用米氏散射理论的共焦偏振显微镜的三维成像分析”Appl.Opt.. 39. 6369-6373 (2000)

Y.Kawata: "Two-stage optical data storage in azo polymers"Jpn.J.Appl.Phys.. 39. 1558-1561 (2000)

Y.Kawata：“偶氮聚合物中的两级光学数据存储”Jpn.J.Appl.Phys.. 39. 1558-1561 (2000)

H.Kitano, M.Murakami, Y.Kawata, C.Egami, O.Sugihara, N.Okamoto, M.Tsuchimori, and O.Watanabe: "Non-optically probing near-field microscopy with the illumination of tatal international reflection"J.Microscopy. (in press).

H.Kitano、M.Murakami、Y.Kawata、C.Egami、O.Sugihara、N.Okamoto、M.Tsuchimori 和 O.Watanabe：“利用全国际反射照明的非光学探测近场显微镜”

Y.Che, O.Sugihara, C.Egami, H.Fujimura, Y.Kawata, N.Okamoto, M.Tsuchimori, and O.Watanabe: "Fabrication of surface reflief grating with second-order nonlinearity using urethaneurea copolymer films"Jpn.J.Appl.Phys.. Vol.38, Part 1, No.11. 6316-6320 (1999)

Y.Che、O.Sugihara、C.Egami、H.Fujimura、Y.Kawata、N.Okamoto、M.Tsuchimori 和 O.Watanabe：“使用聚氨酯脲共聚物薄膜制造具有二阶非线性的表面反射光栅”Jpn

Y.Kawata, C.Egami, O.Nakamura, O.Sugihara, N.Okamoto, M.Tsuchimori, and O.Watanabe: "Non-optically probing nearfield microscopy"Opt.Commun.. Vol.161, No.1. 6-12 (1999)

Y.Kawata、C.Egami、O.Nakamura、O.Sugihara、N.Okamoto、M.Tsuchimori 和 O.Watanabe：“非光学探测近场显微镜”Opt.Commun.. Vol.161，No.1。