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Near-field optical microscopy using an organic thin films as a detection system and its application for the observation of biological specimens

使用有机薄膜作为检测系统的近场光学显微镜及其在生物标本观察中的应用

基本信息

批准号：
13555012
负责人：
KAWATA Yoshimasa
金额：
$ 8.64万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

The near-field scanning optical microscope (NSOM) has been extensively investigated by many researchers, because it is possible with such a microscope to overcome the limitations of optical microscope resolution that are determined by the diffraction limit of light. The NSOMs can observe the refractive index in specimens. This is a unique advantage of NSOMs in comparison with other scanning probe microscopes such as the atomic force microscope (AFM) or the scanning tunneling microscope, which are very powerful tools in the measurement of specimen topography.Since the NSOMs require the scanning time of a probe tip to acquire an image of a specimen, they are difficult to apply to the observation of living specimens or moving biological specimens or very fast phenomena.We have developed a new type near-field microscope using an organic thin film as a detection system of light. In the near-field microscope, the optical field distributions near the specimens are recorded as the surface topography of a photosensitive film, and the topographical distributions are readout with an atomic-force microscope. Since the near-field microscope does not require the scanning of a probe tip for illumination or detection or scattering of light, it is possible to observe moving biological specimens and fast phenomena. We demonstrated the observation of a moving paramecium and euglena gracilis with subwavelength resolution.

近场扫描光学显微镜（NSOM）已被许多研究人员广泛研究，因为它是有可能与这样的显微镜，以克服由光的衍射极限所决定的光学显微镜分辨率的限制。NSOM可以观察样品的折射率。这是NSOM与其他扫描探针显微镜（如原子力显微镜（AFM）或扫描隧道显微镜）相比的独特优势，这些扫描探针显微镜是测量样品形貌的非常强大的工具。由于NSOM需要探针尖端的扫描时间来获取样品的图像，它们很难应用于观察活体标本或移动的生物标本或非常快的现象。我们开发了一种新型的近-场显微镜使用有机薄膜作为光的检测系统。在近场显微镜中，样品附近的光场分布被记录为光敏膜的表面形貌，并且用原子力显微镜读出形貌分布。由于近场显微镜不需要扫描探针尖端进行照明或检测或散射光，因此可以观察移动的生物标本和快速现象。我们演示了一个移动草履虫和眼虫gracilis亚波长分辨率的观察。