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Development of automatic biosubstance recognition system

生物物质自动识别系统开发

基本信息

批准号：
10650399
负责人：
SEKI Kyoshiro
金额：
$ 1.79万
依托单位：
IWATE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650399/
关键词：
biosubstance enzyme reaction thin film vacuum deposition method metal multilayer sputtering method clinical chemistry environment analysis 高温アニーリング臨床化学分析

项目摘要

In this research we developed automatic biosubstance recognition system using the temperature dependency of electric resistance or magnetization of a composite metal oxide thin film which consisted of transition metals such as Fe, Mn, Zn, Ni and etc. The results of this research are shown as following:1) The Fe-Mn-Ni composite metal oxide thin film as a semiconductor thin film was fabricated using the vacuum deposition method, and the temperature coefficient of its electric resistance is equal to the thermistor.2) The Fe-Mn-Zn composite metal oxide thin film (ferri magnetic thin film) was fabricated using sputtering method, and it was confirmed that the magnetization had marked temperature dependency.3) Electrical and magnetic characteristics of that thin films have the sensitivity which can sufficiently detect small enthalpy change with the catalysis of the enzyme.4) The construction of the automatic biosubstance recognition system utilizing the prepared thin films and the automatic b … More iosubstance operation unit purchased for GRANT-IN-AID, and annexperiment on the fixed quantity in concentration of biosubstance samples (D-glucose, urea, etc.) due to enzymes (urease, glucose oxidase, etc.) were carried out. The results showed that peak value of output pulse voltage was proportional lineally to the concentration of each biosubstance. Especially, the high resolution as 1 .67 x 10ィイD1-4ィエD1 mol/l on the urea-urease enzyme reaction was obtained. The output voltage indicated similar waveshape to various concentration in the same sample, however different response was appeared in the rising time and the waveshape of various samples. Therefore, the recognition of the biosubstance in the mixed solution is possible by the shape of the output waveform.5) The recognition system with the Fe-Mn-Zn composite metal oxide thin film as a sensor is based on new principle, namely the pyro magnetic effect. In the system, both a sensing element and a measuring part is combined magnetically, so that handling of instrument can be remarkably improved as compared with conventional system . Less

本研究利用Fe、Mn、Zn、Ni等过渡金属组成的复合金属氧化物薄膜的电阻或磁化强度的温度依赖性，开发了生物物质自动识别系统。研究结果如下：1）采用真空沉积法制备了半导体薄膜Fe-Mn-Ni复合金属氧化物薄膜，其电阻温度系数相等。 2）采用溅射法制备了Fe-Mn-Zn复合金属氧化物薄膜（铁磁性薄膜），并证实其磁化强度具有明显的温度依赖性。3）该薄膜的电学和磁学特性具有足够的灵敏度，可以充分检测酶催化下的微小焓变。4）利用该薄膜构建自动生物物质识别系统使用制备的薄膜和为GRANT-IN-AID购买的自动生物物质操作装置，对酶（尿素酶、葡萄糖氧化酶等）引起的生物物质样品（D-葡萄糖、尿素等）的浓度进行定量的附加实验。结果表明，输出脉冲电压的峰值与各生物物质的浓度成线性正比。特别是，在尿素-脲酶反应中获得了 1 .67 x 10ィイD1-4ィエD1 mol/l 的高分辨率。同一样品中不同浓度的输出电压表现出相似的波形，但不同样品的上升时间和波形出现不同的响应。因此，通过输出波形的形状来识别混合溶液中的生物物质成为可能。5)以Fe-Mn-Zn复合金属氧化物薄膜作为传感器的识别系统基于新原理，即热磁效应。在该系统中，传感元件和测量部分均通过磁性结合，因此与传统系统相比，可以显着改善仪器的操作性。较少的