Development Thermodynamic Model for Enzymatic Reaction in Supercritical COィイD22ィエD2

A new method, rapid expansion from supercritical solution with A nonsolventis reported for forming polymer microparticles containing enzyme. A suspension of protein in carbondioxide(CO D22 - D2) containing a cosolvent and dissolved polymer is sprayed through a nozzle toatmospheric pressure. The solubilities of these polymers in CO - 22 - D2 increase significantly withthe use of small alcohols as cosolvents. Obtained solubility data可以估计by thethermodynamic model.furthermore,structural ring-opening of lactone驱动by enzymatic polymerization has been feasible using lowconcentration dosages of polymer-coated lipases in supercritical(SC)-CO - D22 - D2. By comparison，enzymatic synthesis rate with coated lipase proceeded at a rate 10-fold better than native lipase inD2. The rate of The synthesis with The surfactant-coated lipase was approximately twicein routine organic media, cyclohexane. Furthermore, a higher polymeric molecular weight，better conversion(100%) were obtained following polyesterification tests with surfactant-coatedlipases in SC-CO D22 - D2. The synthesized polymer molecular weight by surfactant-coated lipases inSC-CO - D22 - D2 can be controlled by changing the pressure

A new method, rapid expansion from supercritical solution with a nonsolvent, is reported for forming polymer microparticles containing enzyme. A suspension of protein in carbon dioxide(COィイD22ィエD2) containing a cosolvent and dissolved polymer is sprayed through a nozzle to atmospheric pressure. The solubilities of these polymers in COィイD22ィエD2 increase significantly with the use of small alcohols as cosolvents. Obtained solubility data can be estimated by the thermodynamic model.Furthermore, structural ring-opening of lactone driven by enzymatic polymerization has been feasible using low concentration dosages of polymer-coated lipases in supercritical(SC)-COィイD22ィエD2. By comparison, enzymatic synthesis rate with coated lipase proceeded at a rate 10-fold better than native lipase in SC-COィイD22ィエD2. The rate of the synthesis with the surfactant-coated lipase was approximately twice that in routine organic media, cyclohexane. Furthermore, a higher polymeric molecular weight, and better conversion(100%) were obtained following polyesterification tests with surfactant-coated lipases in SC-COィイD22ィエD2. The synthesized polymer molecular weight by surfactant-coated lipases in SC-COィイD22ィエD2 can be controlled by changing the pressure.