Development Thermodynamic Model for Enzymatic Reaction in Supercritical COィイD22ィエD2

开发超临界 COD22D2 酶促反应的热力学模型

• 批准号: 10650755
• 负责人: MISHIMA Kenji
• 金额: $ 2.24万
• 依托单位: FukuokaUniversity
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650755/
• 关键词: Supercritical Fluid; Carbon Dioxide; Enzyme; Reaction; Thermodynamic; Coating; Encapsulation; Cosolvency; 酵素反応; 超臨界二酸化炭素; 溶解度; タンパク質

A new method, rapid expansion from supercritical solution with a nonsolvent, is reported for forming polymer microparticles containing enzyme. A suspension of protein in carbon dioxide(COィイD22ィエD2) containing a cosolvent and dissolved polymer is sprayed through a nozzle to atmospheric pressure. The solubilities of these polymers in COィイD22ィエD2 increase significantly with the use of small alcohols as cosolvents. Obtained solubility data can be estimated by the thermodynamic model.Furthermore, structural ring-opening of lactone driven by enzymatic polymerization has been feasible using low concentration dosages of polymer-coated lipases in supercritical(SC)-COィイD22ィエD2. By comparison, enzymatic synthesis rate with coated lipase proceeded at a rate 10-fold better than native lipase in SC-COィイD22ィエD2. The rate of the synthesis with the surfactant-coated lipase was approximately twice that in routine organic media, cyclohexane. Furthermore, a higher polymeric molecular weight, and better conversion(100%) were obtained following polyesterification tests with surfactant-coated lipases in SC-COィイD22ィエD2. The synthesized polymer molecular weight by surfactant-coated lipases in SC-COィイD22ィエD2 can be controlled by changing the pressure.

报道了一种新的制备含酶聚合物微粒的方法--非溶剂超临界溶液快速膨胀法。将含有助溶剂和溶解的聚合物的蛋白质在二氧化碳中的悬浮液（CO 22）通过喷嘴喷射至大气压。这些聚合物在CO_（22）所得溶解度数据可由热力学模型估算。此外，在超临界（SC）-CO催化剂D22中，使用低浓度剂量的聚合物包被的脂肪酶，通过酶促聚合驱动内酯的结构开环是可行的。通过比较，在SC-CO酶D22中，用包被的脂肪酶的酶促合成速率以比天然脂肪酶好10倍的速率进行。用表面活性剂包被的脂肪酶的合成速率约为常规有机介质环己烷中的两倍。此外，更高的聚合物分子量，和更好的转化率（100%），获得以下的聚酯化测试与表面活性剂包被的脂肪酶在SC-CO脂肪酶D22中D2。通过改变压力，可以控制在SC-CO催化剂D22中由表面包覆的脂肪酶合成的聚合物的分子量。

项目成果

K.Mishima, K.Matsuyama, Y.Taruta, M.Ezawa, Y.Ito, M.Nagatani: "Production of Polythylene Glycol and Polyoxyalkylene-Alkylphenyl Ether Microspheres Using Supercritical Carbon Dioxide"Studies in Surface and Catalysis. 114. 661-664 (1998)

K.Mishima、K.Matsuyama、Y.Taruta、M.Ezawa、Y.Ito、M.Nagatani：“使用超临界二氧化碳生产聚乙二醇和聚氧化烯烷基苯基醚微球”表面和催化研究。

K. Matsuyama, K. Mishima, Y. Taruta. M. Ezawa, M. Nagatani: "Extraction of Astaxanthin from Silver Nitrate Solution Using Supercritical Fluid Extraction"Solv. Extr. Res. Dev. Japan. Vol. 5. 166-171 (1998)

K.松山，K.三岛，Y.塔鲁塔。

K. Mishima, K. Matusyama, M. Nagatani: "Solubilities of Poly(Ethylene Glycol )s in the Mixture Carbon Dioxide and Cosolvent"Fluid Phase Equilibria. Vol. 161. 315-324 (1999)

K. Mishima、K. Matusyama、M. Nagatani：“聚乙二醇在二氧化碳和共溶剂混合物中的溶解度”流体相平衡。

K.Mishima,K.Matsuyama,M.Nagatani: "Solubilities of Poly(Ethylene Glycol)s in the Mixture Carbon Dioxide and Cosolvent"Fluid Phase Equilibria. 161. 315-324 (1999)

K.Mishima，K.Matsuyama，M.Nagatani：“聚乙二醇在二氧化碳和助溶剂混合物中的溶解度”流体相平衡。

K.Mishima,K.Matsuyama,D.Tanabe,S.Yamauchi,T.J.Young,K.P.Johnston: "Microencapsulation of Proteins by Rapid Expansion of Supercritical Solution with a Non solvent"AIChE J.. (印刷中.

K. Mishima、K. Matsuyama、D. Tanabe、S. Yamauchi、T. J. Young、K. P. Johnston：“通过非溶剂超临界溶液快速膨胀实现蛋白质微胶囊化”AIChE J..（出版中。