Development Thermodynamic Model for Enzymatic Reaction in Supercritical COィイD22ィエD2

一种新的方法，从超临界解决方案中快速扩展，据报道形成含有酶酶的聚合物微粒子。A suspension of protein in carbon dioxide（COイイD22エD2） containing a cosolvent and dissolved polymer is sprayed through a nozzle to atmospheric pressure。The solubilities of these polymers in CO-D22-D2 increase significantly with the use of small alcohols as cosolvents。可获得的可溶性数据可以根据热力学模型估计。Furthermore，由酶聚合物驱动的乳内酯的结构环打开可以使用超临界（SC）-CO D22中聚合物涂覆脂质的低浓度剂量。通过比较，酶合成率与涂布脂酶过程中的速率10-fold better an native脂酶in SC-CO D22-D2。用表面涂层的脂质体合成酶的速率大约在正常有机介质、环己烯中两次。Furthermore，一个高聚合分子重量，更好的转化（100%）在SC-CO中使用表面涂层脂质进行了后续的聚合测试。The synthesized polymer molecular weight by surfactant-coated lipases in SC-CO-D22-D2 can be controlled by changing the pressure。

A new method, rapid expansion from supercritical solution with a nonsolvent, is reported for forming polymer microparticles containing enzyme. A suspension of protein in carbon dioxide(COィイD22ィエD2) containing a cosolvent and dissolved polymer is sprayed through a nozzle to atmospheric pressure. The solubilities of these polymers in COィイD22ィエD2 increase significantly with the use of small alcohols as cosolvents. Obtained solubility data can be estimated by the thermodynamic model.Furthermore, structural ring-opening of lactone driven by enzymatic polymerization has been feasible using low concentration dosages of polymer-coated lipases in supercritical(SC)-COィイD22ィエD2. By comparison, enzymatic synthesis rate with coated lipase proceeded at a rate 10-fold better than native lipase in SC-COィイD22ィエD2. The rate of the synthesis with the surfactant-coated lipase was approximately twice that in routine organic media, cyclohexane. Furthermore, a higher polymeric molecular weight, and better conversion(100%) were obtained following polyesterification tests with surfactant-coated lipases in SC-COィイD22ィエD2. The synthesized polymer molecular weight by surfactant-coated lipases in SC-COィイD22ィエD2 can be controlled by changing the pressure.