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Study on Isolation and Biochemical Examination of Psychrotrophic Bacterium for Large Scale Production of Antimicrobial Violet Pigment

大规模生产抗菌紫色颜料的耐冷菌分离及生化检测研究

基本信息

批准号：
10650780
负责人：
NAKAMURA Yoshitoshi
金额：
$ 2.18万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10650780/
关键词：
violet pigment psychrotrophic bacterium antimicrobial activity putrefactive bacterium natural pigment

项目摘要

About 20 % of all foodstuffs produced in the world are wasted because of septic action by microorganisms and many people have experienced bad health effects due to the ingestion of septic food. Since many microorganisms have been reported as putrefactive and pathogenic bacteria, which cause the septic action of food, the prevention of bacteria contamination in food storage is one of the most important factors for supplying safe and healthy food to the consumer. The authors have screened a psychrotrophic bacterium, RT102 strain, which synthesized an antimicrobial violet pigment, from the intestine of a rainbow trout and found out that the violet pigment shows an antimicrobial action for some species of microorganisms. The chemical structure and antimicrobial activity of violet pigment produced by the psychrotrophic bacterium isolated from the intestine of a rainbow trout were investigated experimentally. The psychrotrophic bacterium was found to be a new species belonging to the genus Janthinobacterium lividum. ィイD11ィエD1H, ィイD113ィエD1C-NMR, and FT-MS spectra analyses results showed that the chemical structure of violet pigment was a mixture of vioracein and deoxyvioracein. The antimicrobial activity of violet pigment was confirmed for putrefactive bacteria such as Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, and Pseudomonas aeruginosa. The growth inhibitory and lethal effects of violet pigment on the putrefactive bacteria were evaluated by increasing the concentration of violet pigment, ranging from 5 to 20 mg/1. It was found that higher concentrations of violet pigment caused not only growth inhibition but also the death of the putrefactive bacteria.

世界上生产的所有食品中约有20%由于微生物的腐败作用而被浪费，并且许多人由于摄入腐败食品而经历了不良的健康影响。由于许多微生物已被报道为引起食品腐败作用的腐败菌和致病菌，因此防止食品储存中的细菌污染是向消费者提供安全健康食品的最重要因素之一。作者从虹鳟鱼肠道中筛选出一株能合成抗菌紫色素的耐冷菌RT 102菌株，并发现该紫色素对某些微生物具有抗菌作用。对从虹鳟鱼肠道中分离的一株嗜冷菌所产紫色素的化学结构和抑菌活性进行了初步研究。该嗜冷菌为一新种，隶属于青金杆菌属。通过对紫色素D11、D113、D1C-NMR和FT-MS的分析，确定了紫色素的化学结构为紫霉素和脱氧紫霉素的混合物。紫色素对枯草芽孢杆菌、巨大芽孢杆菌、金黄色葡萄球菌、铜绿假单胞菌等腐败菌的抗菌活性得到证实。通过增加紫色素的浓度，从5到20毫克/升的范围内的生长抑制和致死效果的腐败细菌的紫色色素进行了评价。结果发现，较高浓度的紫色素不仅导致生长抑制，而且腐败细菌的死亡。