Study on Isolation and Biochemical Examination of Psychrotrophic Bacterium for Large Scale Production of Antimicrobial Violet Pigment

世界上大约有20%的食品产品被微生物和许多人所浪费的食用性行为，因为微生物和许多人都经历了对食用性食品的不良健康影响。自从有许多微生物被报道为超活性和病理性细菌以来，是因为食品的Septic action，防止食品储存中的细菌感染是为消费者供应安全和健康食品的最重要因素之一。作者筛选出了一种精神病细菌、RT 102菌株、一种合成了一种抗生素紫颜料的方法，从彩虹鳟鱼的直觉中发现了一种抗生素活性，表明了一种微生物种类的抗生素。“紫颜料的化学结构和抗菌活动是由精神病细菌孤立产生的，从研究的实验中彩虹鳟鱼的直觉中产生的。”精神病细菌已经被发现是一种属于基因Janthinobacterium lividum的新物种。イD11イエD1H、イD113イエD1C-NMR、and FT-MS spectra analyses results showed that the chemical structure of violet pigment was a mixture of vioracein and deoxyvioracein。紫颜料的抗微生物活动已被确认为诸如枯草芽孢杆菌、大芽孢杆菌、金黄色葡萄球菌和假单胞菌等多毒性细菌。紫色素对乳头状细菌的生长抑制和大影响，由于评估了紫色素的浓度，范围从5至20毫克/1。它发现了一种更高的浓度，因为这种浓度不仅导致了增长抑制，而且还导致了过度活性细菌的死亡。

About 20 % of all foodstuffs produced in the world are wasted because of septic action by microorganisms and many people have experienced bad health effects due to the ingestion of septic food. Since many microorganisms have been reported as putrefactive and pathogenic bacteria, which cause the septic action of food, the prevention of bacteria contamination in food storage is one of the most important factors for supplying safe and healthy food to the consumer. The authors have screened a psychrotrophic bacterium, RT102 strain, which synthesized an antimicrobial violet pigment, from the intestine of a rainbow trout and found out that the violet pigment shows an antimicrobial action for some species of microorganisms. The chemical structure and antimicrobial activity of violet pigment produced by the psychrotrophic bacterium isolated from the intestine of a rainbow trout were investigated experimentally. The psychrotrophic bacterium was found to be a new species belonging to the genus Janthinobacterium lividum. ィイD11ィエD1H, ィイD113ィエD1C-NMR, and FT-MS spectra analyses results showed that the chemical structure of violet pigment was a mixture of vioracein and deoxyvioracein. The antimicrobial activity of violet pigment was confirmed for putrefactive bacteria such as Bacillus subtilis, Bacillus megaterium, Staphylococcus aureus, and Pseudomonas aeruginosa. The growth inhibitory and lethal effects of violet pigment on the putrefactive bacteria were evaluated by increasing the concentration of violet pigment, ranging from 5 to 20 mg/1. It was found that higher concentrations of violet pigment caused not only growth inhibition but also the death of the putrefactive bacteria.