Inheritance of morphological and physiological characters in the inter-subspecific cross between Samia cynthia ricini and S. c. pryeri.

F1代杂种是用蓖麻蚕♀与蓖麻蚕♂杂交而成。F1雌蛹没有进入滞育状态，而F1雄蛹大部分进入滞育状态，这说明蛹的滞育性质是以性连锁的方式遗传的。在回交后代中，蛹的滞育似乎与性有关。在F1代和B1代，在长日光周期下，蛹不表现滞育。在4℃和25℃条件下孵化100天，比较测定其血淋巴中三氯蔗糖的含量和过冷点。在25℃和4℃孵化的普氏苏云金线虫蛹中，海藻糖含量分别为15 mg/ml和34 mg/ml。在25只℃和4只℃孵化的F2代幼虫中，其平均含量分别为14 mg/ml和18 mg/ml。在F2代4只℃孵化的蚕蛹中，个体间海藻糖含量变化范围为5~37 mg/ml。25个℃孵化的蛹平均过冷点为-27℃，4个℃孵化的蛹平均过冷点为-21 Mbps。根据对回交后代的观察，发现青海棠的绿色是由常染色体上的显性基因控制的。普氏链球菌皮肤上的黑点也是由另一个常染色体上的显性基因控制的。普里耶里的茧是棕色的。在B_1代，茧色分离，观察到各种颜色。一些遗传分析让我用一个模型来解释茧的颜色；一个基因(S)和另一个基因(S)分别控制着红色和灰色的色素。

The F1 hybrids were obtained from a cross between Samia cynthia pryeri ♀× Samia cynthia ricini ♂. The F1 female pupae did not enter diapause, whereas most of the F1 male pupae enter diapause, suggesting that the pupal diapause nature is inherited in a sex linked manner. In the backcross progeny, the pupal diapause seemed to be sex linked. In F1 and B1 generations, the pupae did not show diapause under a long day photoperiod. The diapausing pupae of S. c. pryeri and the F2 hybrids were incubated at 4℃ vs. 25℃ for 100 days, and their trahalose content and supercooling point in the hemolymph were measured comparatively. In 25℃- and 4℃-incubated pupae of S. c. pryeri, the trehalose content was 15mg/ml and 34mg/ml, respectively. In 25℃- and 4℃-incubated pupae in the F2 generation, it was 14mg/ml and 18mg/ml (average), respectively. In 4℃-incubated pupae in the F2, trehalose content varied from 5 to 37 mg/ml among individuals. The supercooling point was -27℃ in the 25℃-incubated pupae and -21℃ at 4℃-incubated ones, in average. No correlation was observed between the supercooling point and trahalose content in F2 individuals.Based on my observation of the backcross progenies, it was clarified that the green skin color in S. c. pryeri was controlled by a dominant gene on an autosome. The black spots on the skin of S. c. pryeri was also controlled by a dominant gene on another autosome. The cocoon of S. c, pryeri was brown. The cocoon color was segregated in the B1 generation, and various kind of colors were observed. Some genetical analyses led me to explain the cocoon color by a model ; A gene(s) and another gene(s) control a red pigment and a gray pigment, respectively.