权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Horizontal gene transfers from eubacteria to lepidopeteran insects during evolution : Mechanism and and biological significance.

进化过程中从真细菌到鳞翅目昆虫的水平基因转移：机制和生物学意义。

基本信息

批准号：
14360032
负责人：
SHIMADA Toru
金额：
$ 9.66万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Horizontal or lateral gene transfers between distantly separated species are common in bacteria. In eukaryotes, however, horizontal gene transfers have not been reported with a few exceptions : Mita et al. (2003) reported that several genes found in the Born byx EST database that encode a chitinase (BmChi-h), sucrase (beta-fructofuranosidase), glycerophosphoryl phosphodiesterase (GlpQ), and glucose-l-phosphatase, show higher homology to bacteria than to eukaryotes. Among these, BmChi-h has been mapped to the silkworm chromosome (Daimon et al., 2003). These are candidates for horizontal gene transfer between lepidopterans and bacteria. The enzymatic properties of BmChi-h were further analyzed in this study.Not only are there bacterium-derived genes, but also baculovirus-derived genes in the Born byx and other lepidopteran genomes. Friesen and Nissen (1990) reported the presence of the "TED" element from Trichoplusia ni, which contains three open reading frames flanked by LTRs. The third ORF is homologous to the gene for envelope fusion proteins (e. g., ORF 130 in LdNPV) in Class II baculoviruses. Its structure is similar to Drosophila endogenous retroviruses, including gypsy. We found that not only Trichoplusia but also Bombyx carries retrovirus-like elements. The EST and the whole-genome shotgun sequences seem to contain at least 5 families of retrovirus-like elements, whose final ORFs encode ENV-like proteins.We also found 12 fragmental sequences of genomic DNA that show strong homology to mitochondrial DNA of Bombyx mori. This is the first evidence indocating the Bombyx genome has incorporated mitochondrial DNA.

在遥远的物种之间水平或横向的基因转移在细菌中很常见。然而，在真核生物中，水平基因转移尚未报道，只有少数例外：Mita等人（2003）报道，在Born byx EST数据库中发现的编码几丁质酶（BmChi-h）、蔗糖酶（β -果糖呋喃苷酶）、甘油磷酸基磷酸二酯酶（GlpQ）和葡萄糖-l-磷酸酶的几个基因与细菌的同源性高于与真核生物的同源性。其中，BmChi-h已被定位到家蚕染色体上（damon et al., 2003）。这些是鳞翅目动物和细菌之间水平基因转移的候选者。本研究进一步分析了BmChi-h的酶学性质。鳞翅目基因组中不仅有细菌衍生基因，还有杆状病毒衍生基因。Friesen和Nissen（1990）报道了来自Trichoplusia ni的“TED”元素的存在，它包含三个开放的阅读框，两侧是ltr。第三个ORF与II类杆状病毒的包膜融合蛋白基因同源（例如，LdNPV中的orf130）。它的结构类似于果蝇内源性逆转录病毒，包括吉普赛病毒。我们发现毛虫和家蚕都携带逆转录病毒样成分。EST和全基因组霰弹枪序列似乎包含至少5个逆转录病毒样元件家族，其最终orf编码env样蛋白。我们还发现了12个与家蚕线粒体DNA同源性较强的基因组DNA片段。这是第一个证明家蚕基因组包含线粒体DNA的证据。

项目成果

期刊论文数量（22）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Abe, H., Sugasaki, T., Terada, T., Kanehara, M., Ohbayashi, F., Shimada, T., Kawai, S., Mita, K, Oshiki, T: "Nested retrotransposons on the W chromosome of the wild silkworm Bornbyx mandarins"Insect Mol.Biol.. 11. 307-314 (2002)

Abe, H.、Sugasaki, T.、Terada, T.、Kanehara, M.、Ohbayashi, F.、Shimada, T.、Kawai, S.、Mita, K、Oshiki, T：“W 染色体上的嵌套逆转录转座子

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Kazuei Mita, Mitsuoki Morimyo, Kazuhiro Okano, Yoshiko Koike, Junko Nohata, Hideki Kawasaki, Keiko Kadono-Okuda, Kimiko Yamamoto, Masataka G.Suzuki, Toru Shimada, Marian R.Goldsmith, Susumu Maeda: "The construction of an EST database for Bombyx mori and i

Kazuei Mita、Mitsuoki Morimyo、Kazuhiro Okano、Yoshiko Koike、Junko Nohata、Hideki Kawasaki、Keiko Kadono-Okuda、Kimiko Yamamoto、Masataka G.Suzuki、Toru Shimada、Marian R.Goldsmith、Susumu Maeda：“构建 EST 数据库