Basic studies on physical mapping, transcript mapping, and functional analysis of the silkworm (Bombyx mori) genome

家蚕基因组物理作图、转录作图和功能分析的基础研究

• 批准号: 17018007
• 负责人: SHIMADA Toru
• 金额: $ 53.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17018007/
• 关键词: 比較ゲノム解析; 完全長cDNA; カイコ; クワコ; カイコゲノム; 鱗翅目昆虫; small RNA; ポジショナルクローニング; 伴性遺伝子; SAGE解析; Fosmidライブラリー; 進化; エピジェネティクス; カイコとクワコ; 遺伝子トラップ

We assembled the genome sequences of the silkworm Bombyx mori as international collaboration and published the results. We also performed transcriptome analysis based on the full-length cDNA libraries and small RNA populations, and constructed a database for their sequences. By comparing the B. mori genome with those of other metazoans, we found many Bombyx-specific genes. A part of the specific genes show higher homology to eubacterial genes than to eukaryotic sequences, suggesting horizontal gene transfers from eubacteria to an ancestral lepidopterans. We also compared the B. mori genome with closely-related wild species, Bombyx mandarina and Samia cynthia ricini. On the other hands, we utilized the genomic information for positional cloning of visible mutants in B. mori. As the results, we succeeded in isolations of lem (lemon), rb (red blood), and many other genes.

作为国际合作，我们组装了家蚕的基因组序列，并发表了结果。我们还进行了基于全长cDNA文库和小RNA群体的转录组分析，并构建了它们的序列数据库。通过比较B.与其他后生动物的基因组比较，我们发现了许多家蚕特有的基因。部分特异基因与真核生物相比，与真细菌的同源性更高，表明真细菌的基因水平转移到了鳞翅目昆虫的祖先。我们还比较了B。家蚕基因组与近缘野生种蓖麻蚕和蓖麻蚕的基因组相似。另一方面，我们利用基因组信息定位克隆B中可见的突变体。森。结果，我们成功地分离了lem（柠檬），rb（红血）和许多其他基因。

项目成果

Mutational analysis of active site residues of chitinase from Bombyx mori nucleopolyhedrovirus

• DOI: 10.1016/j.virusres.2006.11.001
• 发表时间: 2007-03-01
• 期刊: VIRUS RESEARCH
• 影响因子: 5
• 作者: Daimon, Takaaki;Katsuma, Susumu;Shimada, Toru
• 通讯作者: Shimada, Toru

Non-molting glossy/shroud encodes a short-chain dehydrogenase/reductase that functions in the 'Black Box' of the ecdysteroid biosynthesis pathway

• DOI: 10.1242/dev.045641
• 发表时间: 2010-06-15
• 期刊: DEVELOPMENT
• 影响因子: 4.6
• 作者: Niwa, Ryusuke;Namiki, Toshiki;Shinoda, Tetsuro
• 通讯作者: Shinoda, Tetsuro

A 25 by-long insertional mutation in the BmVarp gene causes the waxy translucent skin of the silkworm, Bombyx mori

BmVarp 基因中的 25 个长度插入突变导致家蚕 Bombyx mori 的蜡质半透明皮肤

• 发表时间: 2009
• 期刊: Insect Biochemistry and Molecular Biology 39
• 作者: Ito;K.;Katsuma;S.;Yamamoto;K.;Kadono-Okuda;K.;Mita;K.;Shimada;T.
• 通讯作者: T.

Simple sequence repeat-based consensus linkage map of Bombyx mori

• DOI: 10.1073/pnas.0507794102
• 发表时间: 2005-11-08
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Miao, XX;Xu, SJ;Huang, YP
• 通讯作者: Huang, YP

蚕が桑の毒性を回避する仕組み?ゲノムからの謎解き

蚕如何避免桑葚的毒性？从基因组解开谜团？

• 发表时间: 2009
• 作者: Takaaki Daimon;YanMeng;Takeshi Yoshinaga;Susumu Katsuma;Toru Shimada;Toru Shimada;嶋田透
• 通讯作者: 嶋田透