Research and classification of Zn-Bacteriochlorophyll-containing bacteria

含叶绿素锌细菌的研究与分类

• 批准号: 10660069
• 负责人: WAKAO Norio
• 金额: $ 2.18万
• 依托单位: IWATE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660069/
• 关键词: photosynthetic pigments; Zn-bacteriochlorophy11; acidophilic bacteria; Acidiphilium

It has been thought for a long time that Mg-(bacterio)chlorophylls are essential for the photosynthesis in nature. However, Zn-containing bacteriochlorophyll (Zn-BChl) was discovered to be present in the acidophilic aerobic heterotrophic bacteria, genus Acidiphilium, and to be functional in cells. In this study we investigated widely the bacteria with Mg-BChl and/or Zn-BChl in natural acid environments.1. We extracted and analysed the pigments by organic solvents from the acid natural environments such as waters, soils, and biomat. But the pigments could not be detected.2. We tried to detect the puf genes of photosynthetic systems with Mg-BChl and/or Zn-BChl by PCR method but obtained no positive results.3. We collected many acidophilic aerobic heterotrophic bacteria from many acid environments such as acid mine waters, acid hot springs, acid biomat, and analysed the pigments in living cells of the isolates. As the results, relatively many isolates with the Mg-BChl were found but Zn-BChl producers were rarely.

长期以来，人们一直认为Mg-（细菌）叶绿素是自然界光合作用所必需的。然而，含锌的细菌叶绿素（Zn-BChl）被发现存在于嗜酸性好氧异养细菌，嗜酸菌属，并在细胞中发挥作用。本研究对天然酸性环境中的Mg-BChl和/或Zn-BChl细菌进行了广泛的研究.我们采用有机溶剂从酸性自然环境如沃茨、土壤和生物垫中提取并分析了色素。但未检出色素.我们尝试用PCR方法检测Mg-BChl和/或Zn-BChl的光合系统puf基因，但没有得到阳性结果.从酸性矿山沃茨水、酸性温泉、酸性生物垫等酸性环境中采集了大量嗜酸好氧异养菌，并对分离菌株的活细胞色素进行了分析。结果表明，在我国，产Mg-BChl菌株较多，而产Zn-BChl菌株较少。