Form and microbial metabolism of arsenic dissolved in acid mine water and soils.

酸性矿井水和土壤中溶解砷的形态和微生物代谢。

• 批准号: 61560069
• 负责人: WAKAO Norio
• 金额: $ 1.34万
• 依托单位: Iwater University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61560069/
• 关键词: Acid mine water; Arsenic; Arsenite oxidation; Arsenite-oxidizing bacteria; 硫化鉄鉱山; 酸性鉱山水素; 砒素酸化; 砒素の微生物代謝

1. Thionalide method was successfully applied to the differential analysis of soluble arsenic (arsenite and arsenate) in acid mine water form a sulfur-pyrite mine.2. A large amount of arsenic was present in the acid mine water. The arsenic dissolved was predominantly in the form of arsenite (Aslll), but arsenite was rapidly changed to the form of arsenate.3. The transformation of arsenic, arsenite to arsenate, was found to be ascribed to the activity of microorganisms living in the acid mine water.4. Six strains of arsenite-oxidizing bacteria were finally isolated from the habitats and their many phenotypical and chemotaxomonical properties were investigated. As a result, the bacteria were strictly aerobic, acidophilic and heterotrophic bacteria.5. The newly-isolated acidophilic arsenite-oxidizing bacteria were different form the arsenite-oxidizing bacteria hitherto reported, and were thought to be classified as a new genus, Acidiphilium.

1.成功地将硫氰化法应用于某硫硫铁矿酸性矿井水中可溶性砷(亚砷酸盐和亚砷酸盐)的差示分析。酸性矿井水中含有大量的砷。溶解的砷主要以亚砷酸盐(As11)的形式存在，但亚砷酸盐迅速转化为砷的形式。砷从亚砷酸盐向砷酸盐的转化归因于酸性矿井水中微生物的活动。最终从栖息地中分离到6株亚砷酸盐氧化细菌，并对它们的许多表型和化学分类进行了研究。结果表明，该菌为严格的好氧细菌、嗜酸细菌和异养细菌。新分离的嗜酸性亚砷酸盐氧化细菌与已报道的亚砷酸盐氧化细菌不同，被认为是一个新属--嗜酸亚砷杆菌。

项目成果

Norio Wakao: "Microbial oxidation of arsenite and occurrence of arsenite-oxidizing bacteria in acid mine water from a sulfur-pyrite mine." Geomicrobiology Journal. 6. (1988)

Norio Wakao：“亚砷酸盐的微生物氧化以及硫黄铁矿酸性矿水中亚砷酸盐氧化细菌的出现。”

Norio,Wakao: Geomicrobiology Journal. 6. (1988)

Norio，Wakao：地球微生物学杂志。