Researches on photo-utilization of Zn-bacteriochlorophyll-containing phototrophic bacteria

长期以来，人们一直认为Mg-（细菌）叶绿素对自然界的光合作用至关重要。然而，新的光合色素，含锌细菌叶绿素（Zn-BCh1）被发现存在于嗜酸好氧异养细菌中，并在细胞中具有生理功能。本文以红嗜酸菌为类型菌株，对光照条件下光能转化为电能（电流）、化学能（ATP）和还原力（NADPH）的产生以及CO_2的固定进行了研究。在不同的光照条件下，研究了添加介质的电化学电池的输出电流。因此，可以在新鲜细菌细胞中检测到光诱导电流输出。ATP和NADPH的产生与光照强度有关。完整细胞和细胞膜组分的^<14>CO_2分析表明，CO_2在好氧和光照条件下固定。

It has been thought for a long time that Mg-(bacterio) chlorophylls are essential for the photosynthesis in nature. However, the new photosynthetic pigment, Zn-containing bacteriochlorophyll (Zn-BCh1) was discovered to be present in the acidophilic aerobic heterotrophic bacteria, Acidiphilium sp, and to be physiologically functional in cells. The present researches were made, using Acidiphilium rubrum as the type strain, on the conversion of light energy into electrical energy (current generation) via photosynthesis, the productions of chemical energy (ATP) and reducing power (NADPH), and CO_2 fixation under illumination.The current outputs using electrochemical cells added with the mediator (HNQ) under illumination were investigated under various conditions. As a result photo-induced current outputs could be detected in fresh bacterial cells. The productions of ATP and NADPH were dependent on the light intensity. The ^<14>CO_2 analyses using both intact cells and cell membrane fractions revealed the fixation of CO_2 under aerobic and light conditions.