Studies on Germination Stimulants for Root Parasitic Weeds Produced by Root Cultures of Non-host Plants

组织培养技术已经部署了一些植物的能力来生产Striga germination刺激剂。“这些技术是为生长植物水化的第一次使用的，并提供的几项先进技术的。”它们是无实验室的，不需要的空间，允许对环境和限制性问题进行控制，并被微生物捕获。Furthermore，在一些其他二级代谢物的情况下，通过中间成分操纵组织文化，Menispermum dauricum根文化是最有效的生产者的Striga germination刺激物比较了所有测试的植物。最少生产3种活性化合物的文化。高刺激性生产的组织文化是由文化条件的操纵建立的。A modified B5 medium containing 35.7 mM n\gen at a NO_D23_D2_D1-D1/NH_D24_D2_D1+_D1 ratio of 1 ：42， 0.1mM Fe-D12+-D1， 1.0 mM Ca-D12+-D1， 0.55 mM inorganic phosphorus， 0.28 mM inositol， 4.1 μM nicotinic acid， 3.7 μM pyridoxine hydrochloride，14.8 μM盐酸三胺， 1 μM NAA，和4%sucrose可持续的根生长，通过> 30%的时间和增加的根生物量5-折叠刺激生产，与标准B5介质补充3%sucrose和1 μM NAA进行比较。主要的Striga germination刺激生产由M. dauricum根文化显示了类似的色谱行为、类似的紫外线和质量特性，以真实的条纹。确切地说，主要的Striga农场刺激性生产的M.。姜黄根文化是最可能的类固醇或类似类固醇的分子。

Tissue culture techniques were employed to explore capabilities of several plants to produce Striga germination stimulants. The techniques were used for the first time and offered several advantages over the commonly used conventional techniques of growing plants hydroponically. They were less laborious, required less space, allowed control of the environment and eliminated problems caused by microorganisms. Furthermore, as in the case of several other secondary metabolites from tissue cultures by manipulation of medium composition.Menispermum dauricum root cultures were the most efficient producer of Striga germination stimulants compared across all plants tested. The cultures produced at least 3 active compounds. High-stimulant-producing tissue cultures were established by manipulation of culture conditions. A modified B5 medium containing 35.7mM nitrogen at a NOィイD23ィエD2ィイD1-ィエD1/NHィイD24ィエD2ィイD1+ィエD1 ratio of 1 : 42, 0.1mM FeィイD12+ィエD1, 1.0 mM CaィイD12+ィエD1, 0.55 mM inorganic phosphorus, 0.28 mM inositol, 4.1 μM nicotinic acid, 3.7 μM pyridoxine hydrochloride, 14.8 μM thiamine hydrochloride, 1 μM NAA, and 4% sucrose sustained root growth for a longer period and increased root biomass by >30% and stimulant production by 5-fold, in comparison to the standard B5 medium supplemented with 3% sucrose and 1 μM NAA.The major Striga germination stimulant produced by M. dauricum root cultures displayed similar chromatographic behavior, similar UV and mass spectra to authentic strigol. Accordingly the major Striga germination stimulant produced by M. dauricum root cultures was most likely strigol or a strigol-like molecule.