权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Studies on Germination Stimulants for Root Parasitic Weeds Produced by Root Cultures of Non-host Plants

非寄主植物根系培养产生的根部寄生杂草发芽刺激剂的研究

基本信息

批准号：
10660111
负责人：
SUGIMOTO Yukihiro
金额：
$ 2.11万
依托单位：
Arid Land Research Center, Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Tissue culture techniques were employed to explore capabilities of several plants to produce Striga germination stimulants. The techniques were used for the first time and offered several advantages over the commonly used conventional techniques of growing plants hydroponically. They were less laborious, required less space, allowed control of the environment and eliminated problems caused by microorganisms. Furthermore, as in the case of several other secondary metabolites from tissue cultures by manipulation of medium composition.Menispermum dauricum root cultures were the most efficient producer of Striga germination stimulants compared across all plants tested. The cultures produced at least 3 active compounds. High-stimulant-producing tissue cultures were established by manipulation of culture conditions. A modified B5 medium containing 35.7mM nitrogen at a NOィイD23ィエD2ィイD1-ィエD1/NHィイD24ィエD2ィイD1+ィエD1 ratio of 1 : 42, 0.1mM FeィイD12+ィエD1, 1.0 mM CaィイD12+ィエD1, 0.55 mM inorganic phosphorus, 0.28 mM inositol, 4.1 μM nicotinic acid, 3.7 μM pyridoxine hydrochloride, 14.8 μM thiamine hydrochloride, 1 μM NAA, and 4% sucrose sustained root growth for a longer period and increased root biomass by >30% and stimulant production by 5-fold, in comparison to the standard B5 medium supplemented with 3% sucrose and 1 μM NAA.The major Striga germination stimulant produced by M. dauricum root cultures displayed similar chromatographic behavior, similar UV and mass spectra to authentic strigol. Accordingly the major Striga germination stimulant produced by M. dauricum root cultures was most likely strigol or a strigol-like molecule.

采用组织培养技术研究了几种植物产生独脚金种子萌发刺激物的能力。该技术是第一次使用，并提供了几个优势，超过常用的传统技术，种植植物水培。它们不那么费力，需要的空间更小，可以控制环境并消除微生物引起的问题。此外，在其他几个次生代谢产物的情况下，从组织培养物通过操纵介质composition.Menispermum dauricum根培养物是最有效的生产商的独脚金发芽刺激剂相比，在所有的植物测试。培养物产生至少3种活性化合物。通过控制培养条件，建立了高刺激物产生的组织培养物。将含有35.7mM氮的改良B5培养基以1：1的NO：D23：D2：D1-NH3：D1/NH：D24：D2：D1+ NH3：D1比率进行培养。42、0.1mM Fe ~（2+）D_1、1.0mM Ca ~（2+）D_1、0.55mM无机磷、0.28mM肌醇、4.1 μM烟酸、3.7 μM盐酸吡哆醇，与添加3%蔗糖和1 μM NAA的标准B5培养基相比，添加14.8 μM盐酸硫胺素、1 μM NAA和4%蔗糖的B5培养基可使根生长持续时间更长，根生物量增加30%以上，刺激物产量增加5倍。蝙蝠葛根培养物显示出与真实独脚金醇相似的色谱行为、相似的UV和质谱。因此，M.蝙蝠葛根培养物最可能是独脚金醇或独脚金醇样分子。