Analyses of host and environmental factors affecting proliferation of root parasitic plant Shies

分离响应于独脚金寄生的sorpylase基因，并分析其表达。利用抑制性消减杂交技术，共检测到30个基因在S。hermonthica寄生，分离自感病高粱品种Abu 70的根。在从S. hermonthica寄生的高粱品种Tabat和Wad Ahmed。Tabat对S.荷蒙西卡阿布70.研究了水杨酸（SA）和茉莉酸甲酯（MeJA）处理3个高粱品种根系的变化。S.荷monthica寄生诱导了黄瓜根系中的JA响应基因，抑制了SA响应基因。阿布70和塔巴特。相反，在不太敏感的品种瓦德艾哈迈德，S。hermonthica寄生诱导SA反应基因，但轻微诱导JA反应基因。在被寄生的高粱品种叶片中观察到一些基因的系统表达。赫尔蒙席卡。

Sorghum genes responsive to Striga bermonthica parasitism were isolated and their expression was analyzed. Using the suppression subtractive hybridization strategy, 30 genes, up-regulated in response to S. hermonthica parasitism, were isolated from roots of susceptible sorghum cultivar Abu 70. Changes in expression of each of the genes were investigated in roots and leaves obtained from the S. hermonthica-parasitized sorghum cultivars Tabat and Wad Ahmed. Tabat is more susceptible while Wad Ahmed is less susceptible to S. hermonthica than cv. Abu 70. The changes were studied also in roots of the three sorghum cultivars treated with salicylic acid (SA) or methyl jasmonate (MeJA). S. hermonthica parasitism induced JA-responsive genes and suppressed SA-responsive genes in roots of cvs. Abu 70 and Tabat. In contrast, in the less susceptible cultivar Wad Ahmed, S. hermonthica parasitism induced SA-responsive genes but slightly induced JA-responsive genes. Systemic expression of a few genes was observed in leaves of the sorghum cultivars parasitized with S. hermonthica.