Analysis of strigolactone biosynthesis using high-producing plant culture system

利用高产植物培养系统分析独脚金内酯生物合成

• 批准号: 15580091
• 负责人: SUGIMOTO Yukihiro
• 金额: $ 2.37万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580091/
• 关键词: root parasitic plant; strigol; biosynthesis; Menispermum dauricum; inhibitor; tracer; 根寄生雑草; ストライガ; オロバンキ; 発芽刺激物質; 培養根

Cultured roots of Menispermum dauricum produce and exude strigol, which stimulates germination of several species of the root parasitic genera Striga and Orobanche. The stimulant has been considered to be a sesquiterpene, based on its structure. Two pathways for terpenoid biosynthesis in higher plants, the methyl erithritol phosphate (MEP) pathway and the mevalonate pathway, have been established. Investigations into strigol biosynhesis, using cultured roots of M dauricum, showed that fosmidomycin, an inhibitor of MEP pathway, did not influence activity of M. dauricum root culture filtrate on Striga germination. Feeding M.dauricum cultured roots with [1-^<13>C] glucose and [2-^<13>C] mevalonolactone and subsequent monitoring of ^<13>C incorporation into strigol by ^<13>C-NMR spectroscopy showed no signal enrichment. HPLC purified extracts of cultured M. dauricum roots, previously fed with [2-^<14>C] mevalonolactone, displayed no detectable radioactivity in fractions showing high Striga germination inducing activity. It could thus be concluded that strigol is not synthesized by the mevalonate pathway. Strigol may be synthesized, as is abscisic acid, via carotenoids. Involvement of carotenoids in strigol biosynthesis is, currently, under investigation.

蝙蝠葛的培养根产生并分泌独脚金，其刺激根寄生属独脚金和列当的几个物种的萌发。根据其结构，该兴奋剂被认为是一种倍半萜烯。在高等植物中萜类化合物的生物合成有两条途径，甲基赤藓醇磷酸（MEP）途径和甲羟戊酸途径。用培养的蝙蝠葛根研究独脚金醇的生物合成，结果表明，MEP途径抑制剂膦咪霉素对蝙蝠葛的生物合成活性没有影响。蝙蝠葛根培养滤液对独脚金种子萌发的影响。用[1-^<13>C]葡萄糖和[2-^<13>C]甲羟戊酸内酯喂养蝙蝠葛培养的根，随后<13>通过1HC-NMR光谱监测^ C掺入独<13>脚金醇，结果显示没有信号富集。HPLC纯化培养的M.预先用[2- 1C<14>]甲羟戊酸内酯喂养的蝙蝠葛根在显示高独脚金萌发诱导活性的级分中未显示可检测的放射性。因此可以得出结论，独脚金醇不是通过甲羟戊酸途径合成的。独脚金醇可以通过类胡萝卜素合成，脱落酸也是如此。类胡萝卜素参与独脚金醇的生物合成，目前，正在调查中。

项目成果

杉本 幸裕: "根寄生植物の種子発芽を刺激する物質"根の研究. 12,2. 51-56 (2003)

Yukihiro Sugimoto：“刺激根寄生植物种子萌发的物质”Root Research 12,2 (2003)。

Germination stimulants for root parasitic plants

根寄生植物的发芽刺激剂

• 发表时间: 2003
• 期刊: Root Research 12(2)
• 作者: Yukihiro;Sugimoto
• 通讯作者: Sugimoto

Germination strategy of Striga hermonthica involves regulation of ethylene biosynthesis

• DOI: 10.1034/j.1399-3054.2003.00162.x
• 发表时间: 2003-09-01
• 期刊: PHYSIOLOGIA PLANTARUM
• 影响因子: 6.4
• 作者: Sugimoto, Y;Ali, AM;Itai, A
• 通讯作者: Itai, A

根寄生植物の種子発芽を刺激する物質

刺激根寄生植物种子发芽的物质

• 发表时间: 2003
• 期刊: 根の研究 12
• 作者: Hirose J.;Kitabatake N.;Kimura A.;Narita H.;J.HIROSE;Norifumi YASUDA;Yukihiro SUGIMOTO;杉本 幸裕
• 通讯作者: 杉本 幸裕

Norifumi Yasuda: "(+)-Strigol, a witchweed seed germination stimulant, from Menispermum dauricum root culture"Phytochemistry. 62,7. 1115-1119 (2003)

Norifumi Yasuda：“( )-Strigol，一种巫草种子发芽刺激剂，来自北防豆根培养物”植物化学。