Study on the flower color change of morning glory by change of vacuolar pH

花色色素花青素在离体培养条件下，随着pH值的变化而不断发生颜色变化，色素存在于细胞内的液泡pH值的差异是导致花色变化的原因之一。本研究以花瓣液泡pH值为研究对象，建立了花瓣液泡pH值的直接测定方法，并建立了花瓣液泡内离子浓度测定的电生理实验台。除pH电极外，还进行了填充钾离子载体的细胞内微量钾响应电极的制备，测定了蓝色牵牛（Ipomoea tricolor，cv.Heavenly blue）、红色牵牛（Pharbitis nil cv.Scarlet O 'hara）花瓣液泡pH值。红花蕾花瓣液泡pH值在6.6左右，开放花的pH值为7.6，红牵牛花的液泡pH值在6.1左右，它被证明比天空的7.6低得多牵牛花紫色花瓣细胞液泡内钾离子浓度为70 mM，蓝色开放花液泡内钾离子浓度为38花的颜色与液泡中离子浓度的关系很密切，这方面的研究正在进行中。

The flower color pigment anthocyanin changes its color continuously in vitro by the change of pH.One of the the causes of this the color change of the flower variously will be a difference of vacuole pH in which the pigment exists intracellular. This study was done for the purpose of quantitatively clarifying the correlation of vacuole pH and flower color by developing and establishing direct measurement method in this study of petal vacuole pH by the intracellular micro-electrode method.Electrophysiology loboratory-table for the ion concentration measurement in the petal vacuole by the micro-electrode method was constructed. It also carried out besides besides pH electrode preparation of intracellular minute potassium responsibility electrode which filled the potassium ionophore.The vacuolar pH of Blue morning glory (Ipomoea tricolor, cv.Heavenly blue), red Japan morning glory (Pharbitis nil cv.Scarlet O'hara) petals were measured. Though in the red buds petals vacuole pH was about 6.6, the pH value of open flower was 7.6 The vacuolar pH of the red morning glory was about 6.1, and it was proven to be considerably lower than 7.6 of the sky-blue morning glory.Potassium ion concentration of the purple petal cell vacuole of the morning glory was 70 mM.The ivacuolar potassium ion concentration of blue opened flower was 38 mM.The relationship between flower color and ion concentration in the vacuole has much ineterst and the study are under progress.