Development of the safe gene therapy method for single gene disorder without insertion to a host genom

我们已经报道了通过tsSeV/dF-GFP在体内再生细胞质基因转移的造血细胞。但是，转基因蛋白还没有达到半永久性的维持。然后，注意到造血干细胞的自我更新。我们研究了rSeV对体内活性氧和Ca2+动态状态的影响。因此，转基因的长期生存和半永久维持不仅仅是通过抗氧化剂减少活性氧。通过tsSeV/dF-GFP转染骨髓移植小鼠，观察到与Ca2+和Ca2+相关的激素的变化。在tsSeV/dF-GFP转移基因的半永久性维持中，深入探讨了活性氧和Ca2+的关系。

Ｗe had reported that in vivo repopulation of cytoplasmically gene transferred hematopoietic cells by tsSeV/dF-GFP. But, it has not reached in semipermanent maintenance of transgenics protein.Then, to notice the self renewal of hematopoietic stem cells. We investigated how rSeV influenced to reactive oxygen species and the dynamic state of Ca2+ in vivo. As a result, long -term survival and maintaining of transgene semipermanently was not only by decreasing reactive oxygen species with an antioxidant agent. Change of the hormone relevant to Ca2+ and Ca2+ in the gene transferred bone marrow transplant mouse by tsSeV/dF-GFP was seen.In maintenance of semipermanent of the gene transferred by tsSeV/dF-GFP , the relation of not only reactive oxygen species but also Ca2+ was suggested deeply.