Development of the safe gene therapy method for single gene disorder without insertion to a host genom

我们曾报道过通过tsSeV/DF-GFP对胞质内基因转移的造血细胞进行体内再集落。但是，在转基因蛋白的半永久性维持方面还没有达到。其次，要注意造血干细胞的自我更新。我们研究了rSeV对体内活性氧和钙动态的影响。因此，转基因的长期存活和半永久维持不仅仅是通过使用抗氧化剂减少活性氧物种来实现的。观察了tsSeV/DF-GFP转基因骨髓移植小鼠体内与钙离子和钙离子有关的激素的变化，在维持基因半永久性的过程中，进一步揭示了活性氧与钙离子的关系。

Ｗe had reported that in vivo repopulation of cytoplasmically gene transferred hematopoietic cells by tsSeV/dF-GFP. But, it has not reached in semipermanent maintenance of transgenics protein.Then, to notice the self renewal of hematopoietic stem cells. We investigated how rSeV influenced to reactive oxygen species and the dynamic state of Ca2+ in vivo. As a result, long -term survival and maintaining of transgene semipermanently was not only by decreasing reactive oxygen species with an antioxidant agent. Change of the hormone relevant to Ca2+ and Ca2+ in the gene transferred bone marrow transplant mouse by tsSeV/dF-GFP was seen.In maintenance of semipermanent of the gene transferred by tsSeV/dF-GFP , the relation of not only reactive oxygen species but also Ca2+ was suggested deeply.