Development of the safe gene therapy method for single gene disorder without insertion to a host genom

我们曾报道过tsSeV/dF-GFP介导的细胞质转基因造血细胞的体内再增殖。但是，目前还没有达到转基因蛋白的半永久性维持，因此，要注意造血干细胞的自我更新。研究了rSeV对活性氧自由基和Ca ~（2+）动态的影响。因此，转基因的长期存活和半永久性维持不仅仅是通过抗氧化剂减少活性氧。tsSeV/dF-GFP转基因骨髓移植小鼠体内与Ca ~（2+）和Ca ~（2+）相关的激素发生了变化，提示在tsSeV/dF-GFP转基因骨髓移植小鼠体内，不仅与活性氧有关，而且与Ca ~（2+）有关。

Ｗe had reported that in vivo repopulation of cytoplasmically gene transferred hematopoietic cells by tsSeV/dF-GFP. But, it has not reached in semipermanent maintenance of transgenics protein.Then, to notice the self renewal of hematopoietic stem cells. We investigated how rSeV influenced to reactive oxygen species and the dynamic state of Ca2+ in vivo. As a result, long -term survival and maintaining of transgene semipermanently was not only by decreasing reactive oxygen species with an antioxidant agent. Change of the hormone relevant to Ca2+ and Ca2+ in the gene transferred bone marrow transplant mouse by tsSeV/dF-GFP was seen.In maintenance of semipermanent of the gene transferred by tsSeV/dF-GFP , the relation of not only reactive oxygen species but also Ca2+ was suggested deeply.