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Studies on the reproductive ecology of littoral animals using with DNA polymorphism

利用DNA多态性研究滨海动物生殖生态学

基本信息

批准号：
10660169
负责人：
MUNEHARA Hiroyuki
金额：
$ 2.56万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660169/
关键词：
DNA polymorphism microsatellite mtDNA reproductive behavior underwater video camera egg canibalism kin determination hexgramids カジカアイナメ

项目摘要

The present study clarified about the reproductive ecology of some littoral animals as follows.1. Interspecific breedings among three hexagramids were observed with underwater video camera and mtDNA and genomic DNA (calmosurin encording region) polymorphism analyses. The cross breedings did not occurred at random, but only two combination, between male Hexagrammos otakii and female H.octogrammus and between male H.agrammus and female H.octogrammus. All hybrids were female. DNA analyses detected F2 individuals, suggesting F1 hybrids are fertile.2. Primers of amplification of microsatellite DNA polymorphism were designed for paternity determination of Hypopthychus dybowskii. Males of this fish established a spawning territory around sargassum sea wedows. Five primers successfully amplified microsatellite DNA loci. Of them 4 sets of the primers showed high heterooccurrence frequency of 80% over. Relationship among density of fishes, density of spawning substratum and reproductive success is being investigated with the DNA polymorphism.3. A non-copulating sculpin, Hemilepidotus gilberti, produce much apyrene spermatozoa with plural nuclei during spermatogenesis. The function of apyrene sperm were investigated with fluorescent dye and microsatellite DNA polymorphism. It was showed that the sperm played as a broker toward sneaker's eusperm for sperm-competition.4. Primers of amplification of microsatellite DNA polymorphism were designed for cichlids in Lake Tanganyika, Telmatochromis temporalis and Julidochormis ornatus. The analyses resulted that the two species have a unique mating system, helpers and pirates sired with some of guarded eggs by pair.

本研究对部分沿岸的动物的生殖生态学进行了如下阐述.利用水下摄像、线粒体DNA和基因组DNA（calmosurin编码区）多态性分析，观察了三种六角藻的种间短突。杂交后代不是随机发生的，只有两个组合，即雄性大泷与雌性大泷之间的组合和雄性小泷与雌性大泷之间的组合。所有混血儿都是女性。DNA分析检测到F2个体，表明F1杂种可育.设计微卫星DNA多态性扩增引物，用于东北小萤叶甲的亲子鉴定。这条鱼的男性建立了一个产卵领域周围的马尾藻海weeps。5个引物成功扩增出微卫星DNA位点。其中有4对引物的异源出现率高达80%以上。利用DNA多态性研究鱼类密度、产卵基质密度与繁殖成功率的关系.非交配杜父鱼（Hemilepidotus gilberti）在精子发生过程中产生大量无核精子。用荧光染色和微卫星DNA多态性技术研究了无核精子的功能。结果表明，精子对运动鞋的真精子起着经纪人的作用，进行精子竞争。设计了坦噶尼喀湖慈鲷、颞叶Telmatochromis temporalis和装饰朱丽鲷微卫星DNA多态性扩增引物。分析结果表明，这两个物种有独特的交配系统，帮手和海盗与一些被保护的卵成对交配。