Studies on the diversity and creative mechanism of the reproductive mode in copulating teleost fishes

硬骨鱼类交配繁殖方式多样性及创新机制研究

• 批准号: 13660173
• 负责人: MUNEHARA Hiroyuki
• 金额: $ 2.3万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660173/
• 关键词: multi-spawner; single spawner; copulating; maternal care; cottoid; Radulinopsis taranetzi; sperm polymorphism; Hemilepidotus; 繁殖様式

The present studies carried out a ecological and physiological examination on Scorpaeniformes cottoid species, with the focus of the spawning mode and functional morphology of testes as out-adaptive traits for evolution of copulating behavior.1. Histological observation for investigation of on ovarian annual cycle showed that Radulinopsis taranetzi and R. darjevini, maternal care and copulating fishes, are potential multi-spawners. Experimentally, females of these species spawn two times in a breeding season only when the egg masses was taken out of aquarium tank before hatching, but both spawned only one time when the females succeefully protected the first egg mass until hatching. These results suggested that the second clutch of these cottids is for compensatory eggs when the first clutch was eaten during developing.2. Histological observation on testes showed that copulating period and sperm strage system in testicular organ differed between R. taranetzi and R. darjevini. R. tarane … More tzi copulates for six months from January to July, while R. darjevini for ten months from September to July. In the latter fish, testis weight is relatively heavier and sperm is stored more compactly as sperm bunch than those of the former. In addition small chamber, which is enclosed with connective tissues, is provided near the opening of the tip of penis. It is plausible that these morphological differences function to emit much sperm into female genital tract for intensive sperm competion (long copulaing period).3. A non-copulating sculpin, Hemilepidotus gilberti, produce much apyrene spermatozoa with plural nuclei during spermatogenesis. The function of apyrene sperm were investigated with fluorescent dye and microsatellite DNA polymorphism. It was showed that the sperm played as a broker toward sneaker's eusperm for sperm-competition. In addition it is experimentally showed that apyrene spermatozoa have antidispersive function.4. The past studies indicated that copulating cottoids are multispawers while non-copulating cottoids are single spawner. Multi-spawning brought much benefit for copulating individuals but not benefitial for non-copulating fishes. The present study investigated for Radulinopsis species, showed that the above concept may not adaptive for at least maternal care and copulating fishes. Less

本研究对蝎形目卵状体进行了生态学和生理学研究，重点研究了卵状体的产卵方式和功能形态作为交配行为进化的外适应特征。卵巢年周期调查的组织学观察表明，taranetzi和R. darjevini是母性照顾和交配鱼，是潜在的多次产卵者。在实验中，这些物种的雌性在一个繁殖季节只有在孵化前将卵团从水族箱中取出时才会产卵两次，但当雌性成功地保护了第一个卵团直到孵化时，它们都只产卵一次。这些结果表明，这些虫卵的第二窝是在发育过程中吃掉第一窝虫卵的补偿卵。睾丸组织学观察表明，塔兰氏鼠和达氏鼠在睾丸器官的交媾期和精子异体系统方面存在差异。tarane . More zi的交配期为6个月，从1月到7月，而R. darjevini的交配期为10个月，从9月到7月。与前者相比，后者的睾丸重量相对较重，精子以精子束的形式储存得更紧密。另外，在阴茎尖端的开口附近有一个由结缔组织包裹的小腔室。这些形态上的差异可能是为了向雌性生殖道释放大量精子以进行激烈的精子竞争（较长的交配期）。在精子发生过程中，一种非交配的石斑鱼产生了许多具有复数核的单核精子。用荧光染料和微卫星DNA多态性研究了apyrene精子的功能。结果表明，精子在精子竞争中扮演了中间人的角色。此外，实验还表明，无炔精子具有抗分散作用。过去的研究表明，交配的子母体是多卵体，而非交配的子母体是单卵体。多次产卵对交配个体有很大的好处，但对非交配鱼类没有好处。本研究对Radulinopsis物种进行了调查，结果表明上述概念可能不适合至少是母性照顾和交配的鱼类。少

项目成果

宗原弘幸: "血縁と配偶システム"水産生物の性と行動生態(厚生閣恒星社). 33-47 (2003)

Hiroyuki Sohara：“血缘关系和交配系统”水生生物的性别和行为生态学（Koseikaku Kouseisha）33-47（2003）。

Koya, Y., H. Munehara, K. Takano: "Sperm storage and motility in the ovary of the marine sculpin Alcichthys alcicornis (Teleostei : Scorpaeniformes), with internal fametic association"Journal of Experimental Zoology. 292. 145-155 (2002)

Koya，Y.，H. Munehara，K. Takano：“海洋杜父鱼 Alcichthys alcicornis（Teleostei：Scorpaeniformes）卵巢中的精子储存和运动，与内部 Fematic 关联”实验动物学杂志。

Munehara, H., N. Nagai, Y. Narimatsu: "Isolation and characterization of microsatellite markers from the Hypoptychus dybowskii (Pisces, Gasterosteiformes)"Molecular Ecology Note. 2. 70-71 (2002)

Munehara, H., N. Nagai, Y. Narimatsu：“Hypoptychus dybowskii（双鱼座，Gasterosteiformes）微卫星标记的分离和表征”分子生态学笔记。

Hayakawa, Y., H.Munehara: "Obstructive role of the dimorphic sperm in a non-copulatory marine sculpin, Hemi lepidotus gilberti, to prevent other eusperm"Env. Biol. Fish. 64. 419-427 (2002)

Hayakawa, Y., H.Munehara：“二态精子在非交配海洋杜父父鱼 (Hemi lepidotus gilberti) 中的阻碍作用，以阻止其他受精卵”Env.

Narimatsu, Y., H. Munehara: "Territoriality, egg desertion and mating success of a paternal care fish, Hypoptychus dybowskii"Behaviour. 138. 85-96 (2001)

Narimatsu, Y., H. Munehara：“父系护理鱼 Hypoptychus dybowskii 的领地性、卵遗弃和交配成功”行为。