Studies on the diversity and creative mechanism of the reproductive mode in copulating teleost fishes

硬骨鱼类交配繁殖方式多样性及创新机制研究

基本信息

批准号：
13660173
负责人：
MUNEHARA Hiroyuki
金额：
$ 2.3万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660173/
关键词：
multi-spawner single spawner copulating maternal care cottoid Radulinopsis taranetzi sperm polymorphism Hemilepidotus 繁殖様式

项目摘要

The present studies carried out a ecological and physiological examination on Scorpaeniformes cottoid species, with the focus of the spawning mode and functional morphology of testes as out-adaptive traits for evolution of copulating behavior.1. Histological observation for investigation of on ovarian annual cycle showed that Radulinopsis taranetzi and R. darjevini, maternal care and copulating fishes, are potential multi-spawners. Experimentally, females of these species spawn two times in a breeding season only when the egg masses was taken out of aquarium tank before hatching, but both spawned only one time when the females succeefully protected the first egg mass until hatching. These results suggested that the second clutch of these cottids is for compensatory eggs when the first clutch was eaten during developing.2. Histological observation on testes showed that copulating period and sperm strage system in testicular organ differed between R. taranetzi and R. darjevini. R. tarane … More tzi copulates for six months from January to July, while R. darjevini for ten months from September to July. In the latter fish, testis weight is relatively heavier and sperm is stored more compactly as sperm bunch than those of the former. In addition small chamber, which is enclosed with connective tissues, is provided near the opening of the tip of penis. It is plausible that these morphological differences function to emit much sperm into female genital tract for intensive sperm competion (long copulaing period).3. A non-copulating sculpin, Hemilepidotus gilberti, produce much apyrene spermatozoa with plural nuclei during spermatogenesis. The function of apyrene sperm were investigated with fluorescent dye and microsatellite DNA polymorphism. It was showed that the sperm played as a broker toward sneaker's eusperm for sperm-competition. In addition it is experimentally showed that apyrene spermatozoa have antidispersive function.4. The past studies indicated that copulating cottoids are multispawers while non-copulating cottoids are single spawner. Multi-spawning brought much benefit for copulating individuals but not benefitial for non-copulating fishes. The present study investigated for Radulinopsis species, showed that the above concept may not adaptive for at least maternal care and copulating fishes. Less

目前的研究对天蝎座的鸡皮物种进行了生态和身体检查，其重点是产卵模式和睾丸的功能形态，作为交配行为进化的超适应性特征。1。对卵巢年度周期研究的组织学观察结果表明，孕产妇护理和交配鱼类的radulinopsis taranetzi和R. darjevini是潜在的多产产器。在实验上，只有在孵化前将卵子从水族箱中取出时，这些物种的雌性在繁殖季节两次产卵，但两者仅产生了一次，当雌性成功地保护了第一个卵子直到孵化。这些结果表明，当第一个离合器在发育过程中进食时，这些棉布的第二个离合器是用于补偿性鸡蛋。2。在测试上的组织学观察表明，R. taranetzi和R. darjevini之间的结合器官中的分配周期和精子串系统。 R. Tarane…从一月到7月，更多的Tzi交配了六个月，而R. Darjevini从9月至7月共有十个月。在后一种鱼类中，睾丸重量相对重，精子比前者更紧凑。另外，在阴茎尖端的开口附近，还提供了带有连接时间的小腔室。这些形态学差异的作用是合理的，将大量精子散发到女性生殖道中，以进行强化精子竞争（长期配置时期）。3。在精子发生过程中，一种非填充的造型，Hemilepidotus gilberti产生了许多apyrene精子。用荧光染料和微卫星DNA多态性研究了丙烯精子的功能。结果表明，精子扮演着运动鞋的经纪人的精子竞争。另外，实验表明，丙烯丙烯精子具有抗分散功能。4。过去的研究表明，交配的家庭是多源，而无填充的小屋是单一产卵者。多产卵为交配个体带来了很大的好处，但对非填充鱼没有好处。本研究研究了辐射域的物种，表明上述概念可能至少可以适应至少主要的护理和交配鱼类。较少的