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Survey for resource estimation of substrate spawning littoral fishes with using underwater video camera system

水下摄像系统底质产卵沿岸鱼类资源估算调查

基本信息

批准号：
08660217
负责人：
MUNEHARA Hiroyuki
金额：
$ 1.47万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660217/
关键词：
hexagramid Hypoputichus dybowskii microsatellites polymorphism haircrab sculpin

项目摘要

1.The reproductive behavior of Hexagrammos otakii, H.octogrammus and H.agrammus was observed using an underwater video camera system and SCUBA diving. The following results were chiefly obtained.(1) The reproductive behavior of the 3 species was generally similar, so interspecific crossbreeding, in which female H.octogrammus responded to the courtship of male H.otakii. There is a possibility that concrete blocks of a quay loosen the native reproductive isolation.(2) Sneaking followed pair spawning is 11 of the 26 spawnings. Paternity discrimination for 2 egg masses using microsatellite DNA suggested that 84% of all the eggs deposited in the nest were kin to the territorial male, and the remaining 16% were sired by sneakers.2.It was examined which factor influenced to survivorship of the early life stages of a parental care fish, Hypoptychus dybowskii. Earlier spawned eggs underwent higher mortality during embryonic stages. The cause was predation by fishes such as hexagramids based on the observation with SCUBA diving. Analyzes on otolith daily increments suggested that earlier spawned individuals also underwent higher mortality during larval stages. However, earlier spawned individuals could gain larger body size upto adult stage. Survivorship during embryonic stages to spawning state required for seaweed forests.3.The reproductive cycles of hair crab and helmet crab were examined by using the histological observation. These 2 crabs have the similar reproductive cycle, in which consist of oocyte growth, spawning, incubating, molting, and copulating. One cycle of hair crab take at least 2 or 3 years, but that of helmet crab does one year. Females stay under sandy bottom during long incubation period because of defenseless. Exhaustive protest of incubating females is important to reserve the crab resource.

1.利用水下摄像系统和水肺潜水技术，对大泷石斑鱼、长鳍石斑鱼和无鳍石斑鱼的繁殖行为进行了观察。主要取得了以下结果。(1)3种鱼的繁殖行为基本相似，是种间杂交的结果，雌鱼对雄鱼的求偶行为有反应。码头的混凝土块可能会放松本地生殖隔离。(2)在26次产卵中，有11次是在成对产卵后偷偷溜走的。利用微卫星DNA对2个卵群进行亲子鉴定，结果表明：产卵量中84%为领地性雄鱼，16%为潜育性雄鱼。2.研究了影响东北小回鱼早期存活率的因素。早期产卵的卵在胚胎阶段死亡率较高。根据水肺潜水的观察，其原因是六棱鱼等鱼类的捕食。对耳石日增量的分析表明，较早产卵的个体在幼虫阶段也经历了较高的死亡率。但是，早期产卵的个体可以获得更大的身体大小，直到成年阶段。从胚胎期到产卵期的存活率是海藻林所必需的。3.通过组织学观察，研究了大闸蟹和盔蟹的生殖周期。这两种蟹的生殖周期相似，均由卵母细胞生长、产卵、孵化、蜕皮和交配组成。毛蟹的一个周期至少需要2 - 3年，而盔蟹的周期则需要一年。由于没有防御能力，雌性在长时间的潜伏期中呆在桑迪底部。雌蟹在孵化过程中的奋力抗争对保护资源具有重要意义。