权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

IDENTIFICATION AND SYNTHETIC PATHWAY OF THE MATURATION-INDUCING HORMONE IN THE MARINE TELEOST

海洋真骨动物成熟诱导激素的鉴定及合成途径

基本信息

批准号：
10660182
负责人：
MATSUYAMA Michiya
金额：
$ 2.3万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2001
项目状态：
已结题

项目摘要

In this research project, steroid synthetic pathways in the ovarian follicles of the five species of marine teleost, tiger puffer, yellow tail, red seabream, Japanese mackerel, and bambooleaf wrasse, during vitellogenesis and final oocyte maturation (FOM) were investigated.In the vitellogenic ovarian follicles in the tiger puffer, yellowtail, and Japanese mackerel, E2 is synthesized from androstendione (AD) via testosterone (T), while in red seabream and bambooleaf wrasse E2 is synthesized from AD via estrone (E1) but not T. During FOM, in all species the steroidogenic shift from estradiol-17β to 20β-hydroxylated progestin production occurred. Of the steroids produced, 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) and 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) exhibited the greatest effect on germinal vesicle breakdown (GVBD) in vitro in all species. 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) is confirmed as a maturation-inducing hormone (MIH) of the yellowtail. In the tiger puffer, it was shown that 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) was the MIH of this species. On the other hand, in the Japanese mackerel, red seabream, and bamboleaf wrasse, it has been clarified that both 17,20β-P and 20β-S play a role as MIH. Furthermore, the present study clarified the synthetic pathway of the MIH (s) in each species. In all cases, a combination of inactivation of C17, 20-lyase and activation of 20β-hydroxysteroid dehydrogenase in the ovarian follicles is the common enzymatic kinetics in the FOM.

在本研究项目中，研究了五种海洋硬骨鱼：河豚、黄尾、红鳍、竹叶鱼卵泡在卵黄发生和最终卵母细胞成熟过程中的类固醇合成途径。在发生卵黄的河豚、黄尾和竹叶鱼的卵泡中，雄激素(AD)通过睾酮(T)合成E_2，而在红鱼和竹叶中E_2是通过雌酮(E_1)合成的。在FOM中，所有物种的类固醇合成都从辐射雌醇17β转变为20-β羟基孕酮。在所产生的类固醇中，17，20β-二羟基-4-孕烯-3-酮(17，20β-P)和17，20-β，21-三羟基-4-孕烯-3-酮(20β-S)在体外对生发泡破裂的影响最大。17，20β-二羟基-4-孕烯-3-酮(17，20β-P)是黄尾鱼的成熟诱导激素。在虎河豚中，17，20β，21-三羟基-4-孕烯-3-酮(20β-S)是该物种的MIH。另一方面，已证实17，20-β-P和20-β-S在鲱鱼、红鳍和竹叶鱼中都起着MIH的作用。此外，本研究还阐明了MIH(S)在每个物种中的合成途径。在所有情况下，卵泡中C17，20-裂解酶失活和20-β-羟基类固醇脱氢酶激活是FOM中常见的酶动力学。