Overcoming of problem in direct stock assessment by egg production method and its application in the chub mackerel as model species

为了发展和建立用产蛋法直接评估鲭鱼资源的方法，进行了以下研究。1)2）建立了用PAS染色鉴定排卵后卵泡的简便方法，并估算了野生种群的产卵频率。3）分析了卵原细胞和卵母细胞的细胞分裂周期，以及总生殖力的测定模式。（4）基于线粒体DNA分析的雌鱼个体产卵评价。

To develop and establish the direct stock assessment by the Egg Production Method in chub mackerel, following studies have been performed. 1) Development of the new clearing solution for identification of the nucleus in the formalin-preserved oocyte, 2) Development of the simple method to identify the postovulatory follicles by using PAS staining, and estimation of the spawning frequency in the wild stock, 3) Analysis of the cell division cycles in oogonia and oocytes, and mode of the determination of total fecundity, and 4) Evaluation of spawning in individual female fish based on the mitochondrial DNA analysis.