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Ecology and molecular phylogenetic analysis of marine algicidal labyrinthulids

海洋溶藻迷宫类动物的生态学和分子系统发育分析

基本信息

批准号：
10660200
负责人：
SAKATA Taizo
金额：
$ 0.32万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660200/
关键词：
labyrinthulid algicide diatom plaque molecular phylogeny fatty acid DHA rDNA 系統樹滑走運動 18SrDNA Labyrinthuloldes

项目摘要

Labyrinthulids are known to live on the surface of marine macroalgae and aquatic plants and to be causative organisms for eelgrass wasting disease. Furthermore, they are considered to intake organic matters from the diatom or bacteria cells which are lysed extracellularly by their lytic activities. In this study, distirbution, growth response, and molecular phylogeny of marine labyrinthulids were investigated.Algicidal microorganisms were isolated by using double layered agar plates containing diatom cells as plaque-forming organisms from marine macroalgae and aquatic plants. As the expanding plaque-forming organisms, amoeba, labyrinthulids, and filamentous bacteria were observed. Labyrinthulid isolates formed plaques on the diatom double layered agar plates and colonies on the double layered agar plates containing heated bacterial cells. It was clear that phospholipids derived from diatom, bacteria, or serum stimulate colony growth of labyrinthulid isolates. Docosahexaenoic acid (DHA) content was very high (60-70%) in cellular lipids of labyrinthulid isolates.From molecular phylogenetic analysis of labyrinthulids L95-1 and L95-2 based on 18s rDNA sequences, they were found to be close to Labyrinthula sp. AN-1565 (87% homology) and belong to labyrinthulid phylogenetic group.

已知Labelus thulids生活在海洋大型藻类和水生植物的表面上，并且是海草消耗病的病原生物。此外，它们被认为是从硅藻或细菌细胞中摄取有机物质，这些细胞通过其溶解活性在细胞外溶解。本研究以硅藻细胞为噬菌斑形成菌，采用双层琼脂平板法从海洋大型藻类和水生植物中分离出杀藻微生物，并对其分布、生长反应和分子遗传学进行了研究。作为扩大的噬菌斑形成生物体，观察到阿米巴、嗜热菌和丝状细菌。实验结果表明，实验室分离的实验菌在硅藻双层琼脂平板上形成噬菌斑，在含有加热细菌细胞的双层琼脂平板上形成菌落。很明显，来自硅藻，细菌或血清的磷脂刺激嗜热菌分离株的菌落生长。二十二碳六烯酸（DHA）含量很高（60-70%），根据18 SrDNA序列分析，L95-1和L95-2与Labelthula sp. AN-1565的同源性为87%，属于亚门类。