Study of effects of oxidative stress on tumorigensis in LEC rats that shows a high incidence of spontaneous liver cancer.

氧化应激对 LEC 大鼠致瘤影响的研究表明自发性肝癌的发病率很高。

基本信息

  • 批准号:
    10660289
  • 负责人:
  • 金额:
    $ 1.79万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1998
  • 资助国家:
    日本
  • 起止时间:
    1998 至 2000
  • 项目状态:
    已结题

项目摘要

We investigated effects of oxidative stress on tumorigensis in LEC rats that shows a high incidence of spontaneous liver cancer.1. Although copper and iron accumulated in the liver of LEC rats in an age-dependent manner, we showed that copper accumulation in the liver of LEC rats induced DNA strand breaks, but accumulation of iron did not.2. The slow repair of potentially lethal damage (PLD) occurred in LEC rat cells but not the fast repair of PLD.Wortmannin, an inhibitor of DNA-dependent protein kinase (DNA-PK) enhances the radiosensitivity of control rat cells but not that of LEC rat cells. Although oxidative stress produces a variety of lesions in DNA, double-strand breaks (DSBs) appear to be responsible for most oxidative stress-induced cell death. Repair of DSBs plays an important role in the fixation of fast-repairing PLD.DNA-PK that is a heterotrimeric complex comprised of two components : a large catalytic subunit (DNA-PKcs) and DNA-targeting component (Ku 70 and Ku 80). In the control cells Ku proteins accumulated in the nucleus after production of DSBs, but did not in LEC rat cells.3. LEC rat cells showed an abnormal accumulation of G2/M-phase cells after treatment with oxidative stress at S phase. LEC rat cells also showed a higher sensitivity to induction of apoptosis after treatment of the cells with a variety of stress.These results suggest that a deficiency of DSBs repair and an abnormality of some signal transduction pathways following oxidative stress may be associated with a high incidence of liver cancer of LEC rats.
我们研究了氧化应激对LEC大鼠肿瘤发生的影响,该大鼠显示自发性肝癌的高发病率。1.虽然铜和铁在LEC大鼠肝脏中的积累具有年龄依赖性,但我们发现LEC大鼠肝脏中铜的积累会引起DNA链断裂,而铁的积累则不会。2. LEC 大鼠细胞中发生潜在致命损伤 (PLD) 的缓慢修复,但 PLD 的快速修复不发生。DNA 依赖性蛋白激酶 (DNA-PK) 抑制剂 Wortmannin 增强对照大鼠细胞的放射敏感性,但不增强 LEC 大鼠细胞的放射敏感性。尽管氧化应激会在 DNA 中产生多种损伤,但双链断裂 (DSB) 似乎是大多数氧化应激诱导的细胞死亡的原因。 DSB 的修复在快速修复 PLD.DNA-PK 的固定中起着重要作用,PLD.DNA-PK 是一种异源三聚体复合物,由两个组件组成:大催化亚基 (DNA-PKcs) 和 DNA 靶向组件(Ku 70 和 Ku 80)。在对照细胞中,Ku蛋白在DSB产生后在细胞核中积累,而在LEC大鼠细胞中则没有。 3. LEC大鼠细胞在S期氧化应激处理后表现出G2/M期细胞的异常积累。 LEC大鼠细胞经多种应激处理后,对诱导细胞凋亡也表现出更高的敏感性。这些结果表明,氧化应激后DSB修复的缺陷和某些信号转导通路的异常可能与LEC大鼠肝癌的高发有关。

项目成果

期刊论文数量(56)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
M.Hayashi: "Higher sensitivity in induction of apoptosis in fibroblast cell lines derived from LEC strain rats to UV-irradiation."J.Vet.Med.Sci.. 60. 207-212 (1998)
M.Hayashi:“来自 LEC 品系大鼠的成纤维细胞系对紫外线照射诱导细胞凋亡的敏感性更高。”J.Vet.Med.Sci. 60. 207-212 (1998)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
D.Endoh: "Retroviral sequence located in border region of short unique region and short terminal repeat of Md5 strain Marek's disease virus type 1."J.Vet.Med.Sci.. 60. 227-235 (1998)
D.Endoh:“逆转录病毒序列位于马立克氏​​病病毒 1 型 Md5 株的短独特区和短末端重复的边界区域。”J.Vet.Med.Sci. 60. 227-235 (1998)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
M.Hayashi: "Abnormal accumulation of G2/M-phase cells from LEC strain rats after X-irradiation at S phase."J.Vet.Med.Sci.. 61. 975-978 (1999)
M.Hayashi:“LEC 品系大鼠在 S 期 X 射线照射后 G2/M 期细胞的异常积累。”J.Vet.Med.Sci.. 61. 975-978 (1999)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
K.O.Chao: "Seropretvalence of bovine immunodeficiency virus in dairy and beef cattle herds in Korea."J.Vet.Med.Sci.. 61. 549-551 (1999)
K.O.Chao:“韩国奶牛和肉牛群中牛免疫缺陷病毒的血清预防价。”J.Vet.Med.Sci.. 61. 549-551 (1999)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
M.Hayashi: "Wortmannin, a radiation sensitizer, enhances the radiosensitivity of WKAH rat cells but not that of LEC rat cells."J.Vet.Med.Sci.. 62. 191-194 (2000)
M.Hayashi:“Wortmannin 是一种放射增敏剂,可增强 WKAH 大鼠细胞的放射敏感性,但不会增强 LEC 大鼠细胞的放射敏感性。”J.Vet.Med.Sci.. 62. 191-194 (2000)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

HAYASHI Masanobu其他文献

HAYASHI Masanobu的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('HAYASHI Masanobu', 18)}}的其他基金

Study on Stress-response pathway in tumorigenesis in LEO rats that shows a high incidence of spontaneous liver cancer.
LEO大鼠肿瘤发生中的应激反应通​​路研究显示自发性肝癌的高发生率。
  • 批准号:
    13660305
  • 财政年份:
    2001
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Study on abnormality of DNA recombination in LEC strain rat
LEC品系大鼠DNA重组异常的研究
  • 批准号:
    07660409
  • 财政年份:
    1995
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation of the genes associated with tumorigenicity of Marek's disease virus.
研究与马立克氏病病毒致瘤性相关的基因。
  • 批准号:
    02660301
  • 财政年份:
    1990
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

A novel treatment for REBOA complications: Hydrogen gas inhalation therapy to alleviate oxidative stress due to ischemia-reperfusion injury
REBOA并发症的新型治疗方法:氢气吸入疗法减轻缺血再灌注损伤引起的氧化应激
  • 批准号:
    23K21458
  • 财政年份:
    2024
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Developing trimester-specific placenta organ-on-chips to model healthy and oxidative stress and inflammation-associated pathologies
开发妊娠期特异性胎盘器官芯片来模拟健康和氧化应激以及炎症相关的病理学
  • 批准号:
    10732666
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
Oxidative Stress and Mitochondrial Dysfunction in Chemogenetic Heart Failure
化学遗传性心力衰竭中的氧化应激和线粒体功能障碍
  • 批准号:
    10643012
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
LRRK2 and oxidative stress in Parkinson’s disease
LRRK2 与帕金森病的氧化应激
  • 批准号:
    10799999
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
Investigation of the effects of macrolide antimicrobial agents on oxidative stress tolerance in trophoblast cells
大环内酯类抗菌药物对滋养层细胞氧化应激耐受性影响的研究
  • 批准号:
    23K08863
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
IntBIO: Collaborative Research: Integrating nanobiotechnologies to understand the role of nitro-oxidative stress in the coral-dinoflagellate mutualistic symbiosis dynamics
IntBIO:合作研究:整合纳米生物技术来了解硝基氧化应激在珊瑚-甲藻互利共生动态中的作用
  • 批准号:
    2316389
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Standard Grant
Determining the role of Rac1 palmitoylation in cardiac hypertrophy and oxidative stress
确定 Rac1 棕榈酰化在心脏肥大和氧化应激中的作用
  • 批准号:
    10534386
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
Racial disparities of open angle glaucoma: A study of mitochondria and oxidative stress in human trabecular meshwork
开角型青光眼的种族差异:人类小梁网线粒体和氧化应激的研究
  • 批准号:
    10735655
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
Structural systems biology of microenvironmental oxidative stress and synthetic biology intervention
微环境氧化应激的结构系统生物学与合成生物学干预
  • 批准号:
    10715112
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
Placental identified NHIP regulating neuronal oxidative stress in autism
胎盘发现 NHIP 调节自闭症神经元氧化应激
  • 批准号:
    10717990
  • 财政年份:
    2023
  • 资助金额:
    $ 1.79万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了