Studies on pathogenesis, prevention and treatment of serious diseases.

重大疾病发病机制、防治研究。

• 批准号: 10660302
• 负责人: HAYASHI Toshiharu
• 金额: $ 1.92万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660302/
• 关键词: lactic dehydrogenase virus; equine herpes virus; Ibaraki virus; epizootiology; Th1 cell; cloning; reovirus; gene; インスリン依存型糖尿病; 自己免疫疾患; マウスモデル; レオウイルス2型; 膵島細胞破壊; IFN-Υ

Summary consisted of five items listed bellow.1. Reovirus type-2 (Reo-2) infection in DBA/1 mice causes insulitis, which leads to pancreatic islet-cell destruction, resulting in a diabetes-like syndrome, and we suggested that Reo-2-triggerd autoimmune insulitis may be mediated by helper T(Th)1 cells and interferon-T may play a role in islet inflamation leading to islet cell destruction.2. The effect of lactic dehydrogenase virus (LDV) infection on the protective immune response to the nematode Nippostrongylus brasiliensis and allergic eosinophil reaction were studied. The results suggest that LDV infection modulates protective immune responses against N. brasiliensis by the activation of Th1 cells, and suppression of IL-5 gene expression by LDV infection may not be mediated by a mutual inhibitory mechanism between Th1 and Th2 cells.3. The dissemination of equine herpesvirus type 1 (EHV-1) and -4 among horses in Japan was demonstrated by a type-specific Enzyme-Linked Immunosorbent Assay … More (ELISA). And we indicated that this ELISA using paired sera is useful for a diagnosis and epizootiological study on EHV-1 and -4 infections not only in unvaccinated horses, but also in vaccinated horses in Japan.4. Little information is available on the genetics and molecular properties of Ibaraki virus. In this study we clarified the complete nucleotide sequence of M5 RNA segment encoding VP5 of Ibaraki virus. The M5 segment is 1641 base pairs long with the single open reading frame which predics a polypeptide of 527 amino acids. Also the EHDV-2JPN L2 gene is 3002 base pairs long with a single open reading frame of 2949 bp which predicts a polypeptide of 982 amino acid residues.5. The chick has been considered as the natural host for chicken anemia virus (CAV). We examined chicken, Japanese quail, pigeon, duck and crowsera by a micro-scale serum neutralization test for the presence of virus-neutraizing (VN) antibodies to CAV. As the results, quail might be one of the hosts of CAV-like virus. This is the first report of VN antibodies to CAV in a species other than chicken. Less

摘要包括以下五个项目。结论：1. DBA/1小鼠感染呼肠孤病毒2型（Reo-2）引起胰岛炎，导致胰岛细胞破坏，导致糖尿病样综合征，提示Reo-2-破坏性自身免疫性胰岛炎可能由辅助性T（Th）1细胞介导，干扰素T可能在胰岛炎症导致胰岛细胞破坏中发挥作用.本文研究了乳酸脱氢酶病毒（LDV）感染对巴西日本圆线虫保护性免疫反应和嗜酸性粒细胞变态反应的影响。结果表明，LDV感染调节了针对N. LDV通过激活Th 1细胞而抑制IL-5基因表达，可能不是通过Th 1和Th 2细胞之间的相互抑制机制介导的.在日本，马疱疹病毒1型（EHV-1）和4型在马中的传播是通过型特异性酶联免疫吸附试验证明的 ...更多信息 （ELISA）。本研究表明，在日本，使用配对血清的ELISA不仅可用于未免疫马的EHV-1和EHV-4感染的诊断和流行病学研究，而且可用于免疫马的EHV-1和EHV-4感染的诊断和流行病学研究.关于茨木病毒的遗传学和分子特性的信息很少。本研究阐明了茨木病毒M5 RNA编码区VP 5的全序列。M5片段长1641个碱基对，具有单个开放阅读框，预测527个氨基酸的多肽。EHDV-2 JPN L2基因长3002个碱基对，具有2949 bp的单个开放阅读框，预测了982个氨基酸残基的多肽。鸡一直被认为是鸡贫血病毒（chicken anemia virus，CAV）的天然宿主。我们检查了鸡，日本鹌鹑，鸽子，鸭和乌鸦血清的存在下，病毒中和（VN）抗体的CAV的微量血清中和试验。因此，鹌鹑可能是CAV样病毒的宿主之一。这是首次在鸡以外的物种中发现抗CAV的VN抗体。少

项目成果

Morimoto,M.: "Lactic dehydrogenase virus infection inhibits allergic eosinophil and IL-5 gene expression in vivo"Int.Arch.Aller.Immunol.. 120. 78-84 (1999)

Morimoto,M.：“乳酸脱氢酶病毒感染抑制体内过敏性嗜酸性粒细胞和 IL-5 基因表达”Int.Arch.Aller.Immunol.. 120. 78-84 (1999)

Morimoto, M., Iwata, H. and Hayashi, T.: "Lactic dehydrogenase virus infection inhibits allergic eosinophil and IL-5 gene expression in vivo."Int. Arch. Aller. Immunol.. 120. 78-84 (1999)

Morimoto, M.、Iwata, H. 和 Hayashi, T.：“乳酸脱氢酶病毒感染抑制体内过敏性嗜酸性粒细胞和 IL-5 基因表达。”Int。

Pereira, E.M.N., Nishida, T., Tokubaga, R., Iwata, H. and Inoue, T.: "Cloning and expression of the M5 RNA segment encoding outer capsid VP5 of epizootic hemorrhagic disease virus Japan serotype 2, Ibaraki virus."J. Vet. Med. Sci.. 62(in press). (2000)

Pereira, E.M.N.、Nishida, T.、Tokubaga, R.、Iwata, H. 和 Inoue, T.：“编码流行性出血性疾病病毒日本血清型 2（茨城病毒）外衣壳 VP5 的 M5 RNA 片段的克隆和表达。”

Hayashi, T., Morimoto, M., Iwata, H. and Onodera, T.: "Interferon-plays a role in pancreatic islet-cell destruction of reovirus type 2-induced diabetes-like syndrome in DBA/1 suckling mice."Int. J. Exp. Path.. 79. 313-320 (1998)

Hayashi, T.、Morimoto, M.、Iwata, H. 和 Onodera, T.：“干扰素在 DBA/1 乳鼠中 2 型呼肠孤病毒诱导的糖尿病样综合征的胰岛细胞破坏中发挥作用。”

Pereira, E.M.N., Iwata, H. and Inoue, T.: "The complete nucleotide sequence of segment L2 of Ibaraki virus encoding for the antigen recognized by neutralizing antibodies."J. Vet. Med. Sci.. 62(in press). (2000)

Pereira, E.M.N.、Iwata, H. 和 Inoue, T.：“茨城病毒 L2 片段的完整核苷酸序列，编码中和抗体识别的抗原。”J.