Water in Sciatic Nerve Measured by Double-Quantum-Filtered NMR

通过双量子过滤 NMR 测量坐骨神经中的水分

• 批准号: 10670046
• 负责人: SEO Yoshiteru
• 金额: $ 2.05万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670046/
• 关键词: Water; Quadrupolar interaction; Diffusion; Multiple-quantum NMR; PFG NMR; Degeneration; Intracellular compartmentation; 末梢神経; 座骨神経; パルス磁場勾配NMR法

The Aims of this project is 1) to reach an understanding of factors governing the NMR relaxation of water in peripheral nerve tissue and 2) to separate water compartmentation based on parameters of H-2 double-quantum relaxation to follow "order" structure of water and to use it to understand physiological processed in healthy and pathological tissues.1) Compartmentation of water in sciatic nerve of rats : Three compartments of ィイD12ィエD1HィイD22ィエD2O in anisotropic motion condition were detected by H-2 DQF NMR. The observed residual quadrupole splitting were ca. 480, 125 and 9 Hz at 24℃ which were assigned as ィイD12ィエD1HィイD22ィエD2O in endoneurium, epineurium, and axons respectively. The three DQF signals showed anisotropic diffusion, but only the axoplasm compartment represented restricted diffusion. From restricted diffusion of the axoplasm water, the inner-diameter of axon was estimated as 7.5 um.2) Development of flow-cell type NMR probe : We have made a flow-cell type of H-2 NMR probe. Using a glass capillary (1.5 mm inner diameter), perfusate of the sciatic could be switched within 10 sec which allows us to measure uni-directional water exchange between tissue compartments.3) Assessment of degeneration of sciatic nerve : Changes in water exchange and H-2 DQF relaxation parameters were measured on the Wallerian degeneration nerve. Water permeability increased and water structures in the 3 compartments degenerated by 1-2 weeks. It is possible to follow the degeneration process by H-2 DQF NMR.

本项目的目的是：1）了解周围神经组织中水的NMR弛豫的控制因素; 2）根据H-2双量子弛豫参数分离水区室，以遵循水的“有序”结构，并利用它来了解健康和病理组织中的生理过程。1）大鼠坐骨神经中水的区室：用H-2 DQF NMR确定了在各向异性运动条件下的三个隔室-D12--D1 H--D22--D2 O。观察到的剩余四极分裂是ca。在24℃时，480、125和9 Hz分别在神经内膜、神经外膜和轴突中指定为D12-D1 H-D22-D2 O。三个DQF信号显示各向异性扩散，但只有轴浆室代表限制扩散。2）流动池型核磁共振探针的研制：我们研制了一种流动池型的H-2NMR探针。使用玻璃毛细管（1.5mm内径），坐骨神经的灌注液可以在10秒内转换，这允许我们测量组织区室之间的单向水交换。3）坐骨神经变性的评估：在沃勒变性神经上测量水交换和H-2DQF松弛参数的变化。1-2周时，水渗透性增加，3个隔室中的水结构退化。通过H-2DQF NMR可以跟踪降解过程。

项目成果

Y.Seo: "Anisotropic and restricted diffusion of water in sciatic nerve : A ^2H double quantum filtered NMR study." Magn.Reso.Med.(in press).

Y.Seo：“坐骨神经中水的各向异性和受限扩散：^2H 双量子过滤 NMR 研究。”

Seo, Y. et al.: "Anisotropic and restricted diffusion of water in sciatic nerve : A^2Hdouble-quantum-filtered NMR study"Magn. Reso. Med.. 42. 461-466 (1999)

Seo, Y. 等人：“坐骨神经中水的各向异性和受限扩散：A^2H 双量子过滤 NMR 研究”Magn。

Y.Sharf: "Mapping strain on blood vessel walls using deuterium double-quantum-filtered MRI." Proc.Natl.Acad.Soc.USA. 95. 4108-4112 (1998)

Y.Sharf：“使用氘双量子过滤 MRI 绘制血管壁应变图。”

Seo,Y., Takamiya,H., Ishikawa,H., Nakashima,T., Sharf,Y. & Navon,G.: "Spatially Resolved Magnetic Resonance"P.Bluemler, B.Bluemich, R.Botto, E.Fukushima edited, Wiley-VCH NMR imaging of rigid biological tissues. 445-457 (1998)

Seo，Y.，高宫，H.，石川，H.，中岛，T.，Sharf，Y.

Seo,Y., Shinar,H., Morita,Y. & Navon,G.: "Anisotropic and restricted diffusion of water in sciatic nerve : A ィイD12ィエD1H double-quantum-filtered NMR study"Magn.Reso.Med.. 42. 461-466 (1999)

Seo, Y.、Shinar, H.、Morita, Y. 和 Navon, G.：“坐骨神经中水的各向异性和受限扩散：双量子过滤 NMR 研究” Magn Reso。 -466 (1999)