Analysis of bulk flow in the ceiebral interstitial space measured by H-1 MRI

H-1 MRI 测量的大脑间隙总体流量分析

• 批准号: 18590209
• 负责人: SEO Yoshiteru
• 金额: $ 2.55万
• 依托单位: Dokkyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590209/
• 关键词: water metabolism; MRI; bulk flow; relaxation reagent

Bulk flow of water in the interstitial spars of brain of the rat was analyzed by using H-1 MRI. Several relaxation reagents (Mn ion, Gd-DTPA, polylysine-DTPA-Gd, NMS-60) were injected in the brain with 1-3 uL of volume. Movements of realaxation reagents by the convection of water can be detected by T1-weighted MRI imaging with 1-min interval. In the adult rat brain, when relaxation reagent applied in the frontal side of the cortex, the reagent moved to the nasal direction. On the other hand, when the reagent apply in the tail side of the carte, the reagent moved to the tail direction. In order to minimize the effects of injection of relaxation reagents, we have tried a minimal injection less tah 1 uL However; in the MRI measurements, all metalic apparatus, such as pump, have to take distance from the super conducting magnet. Therefore, the tubing line of the injection become 1 meter in length. Thus, we could not get good reproducibility of the minimal volume injection. We will keep to improve injection method. We also established in vivo imaging system for mice in the 7 T high-field MRI This system is capable to measure newborn and infants of rats. Preliminary results suggest that BBB in the rats infants (day 4) might be leaky than that of adult.

用H-1MRI分析大鼠脑间质间质中的体液流量。将几种松弛试剂(锰离子、Gd-DTPA、聚赖氨酸-DTPA-Gd、NMS-60)按1-3ul的体积注入脑内。通过T1加权MRI成像，可以检测到水对流引起的再松弛试剂的移动，间隔1分钟。在成年大鼠大脑中，当松弛试剂应用于大脑皮层的额侧时，试剂向鼻腔方向移动。另一方面，当试剂涂抹在点菜的尾部时，试剂向尾部方向移动。为了最大限度地减少松弛试剂注入的影响，我们尝试了一种最小注入的方法，但在MRI测量中，所有的金属设备，如泵，都必须与超导磁体保持距离。因此，注入的管路变得1米长。因此，我们不能获得良好的重复性的最小体积注射。我们将继续改进注射方法。我们还在7T高场MRI上建立了小鼠的活体成像系统，该系统能够测量大鼠的新生和幼年。初步结果表明，幼鼠(第4天)的血脑屏障可能比成年大鼠的血脑屏障更容易渗漏。

项目成果

Physiological application of MR microscopy

磁共振显微镜的生理应用

• 发表时间: 2007
• 作者: Y;Seo
• 通讯作者: Seo

Lateral diffusion of Mn^<2+> ion in the brain determined by T_1 relaxation time measured by ^1H MRI

通过 ^1H MRI 测量的 T_1 弛豫时间确定 Mn^<2> 离子在大脑中的横向扩散

• 发表时间: 2007
• 作者: Y;Seo H;Wakamatsu Y;Imaizumi M;Yokoi A;Takamata T;Ogino M;Murakami
• 通讯作者: Murakami

MRマイクロスコピーで迫る生理学

通过 MR 显微镜研究生理学

• 发表时间: 2007
• 作者: Misaki Naoko;et. al.;瀬尾 芳輝
• 通讯作者: 瀬尾 芳輝

Lateral diffusion of Mn^<2+>ion in the brain determined by T_1 relaxation time measured by^1 H MRI

通过 ^1 H MRI 测量的 T_1 弛豫时间确定 Mn^<2> 离子在大脑中的横向扩散

• 发表时间: 2007
• 作者: Y Seo;H Wakamatsu;Y. Imaizumi;M Yokoi;A Takamata;T Ogino;M Murakami
• 通讯作者: M Murakami

Chapter 8. MR microimaging from animal to cell, Non-invasive and Visualization Technologies

第 8 章：从动物到细胞的 MR 显微成像、非侵入性和可视化技术

• 发表时间: 2007
• 期刊: edited by S. Ogawa & T. Ueno, NTS 1080
• 作者: Y;Seo;Unit;I
• 通讯作者: I