Development of Multiple Quantum NMR Spectroscopy and Imgaing : Imaging Application on Bound Water in Biological Systems.

本项目的目标是开发基于多量子核磁共振的新型功能性磁共振成像和跟踪水的有序结构的光谱成像，并利用它来了解健康和病理组织的生理过程。1)双量子滤光片核磁共振成像的发展：我们引入了H-1层选择性双量子滤光片核磁共振成像(H-1 DQF MRI)，它选择性地描绘生物组织中有序的水(结合水)，并发展了H-1和H-2光谱双量子滤光片核磁共振成像(H-1和H-2 DQF CSI)，通过分子动力学的选择窗口描绘了水在特定分子环境中的情况。2)可变角度负载池H-2/H-1探针的研制：为了直观地显示结合水的宏观有序性，有必要测量驰豫时间和在静磁场作用下不同角度的剩余四极体(或偶极体)分裂。3)H-2/H-1DQF CSI对关节软骨中胶原结构的可视化：在完整的牛关节软骨中，我们可以描绘出浅层、切向、深层和钙化区的胶原结构，对胶原结构在静态加载下的变形进行观察和分析，可能在2001年完成。

The Aims of this project is development of new functional MR imaging based on the multiple quantum NMR and the spectroscopic imaging to follow "order" structure of water and to use it to understand physiological processes in healthy and pathological tissues.1) Development of double-quantum-filter NMR imaging : We have introduced H-1 slice selective double-quantum-filter NMR imaging (H-1 DQF MRI) which depicted ordered water (bound water) in biological tissues selectively, and also developed H-1 and H-2 spectroscopic double-quantum-filter NMR imaging (H-1 and H-2 DQF CSI) which depict water in a certain molecular environment through selective window of molecular dynamics.2) Development of variable-angle loaded-cell H-2/H-1 probe : In order to visualize macroscopic order of the bound water, it is necessary to measure relaxation time and residual quadrapoler (or dipoler) splitting in various angle against the static magnetic field. We have built the world-first variable-angle loaded-cell probe for H-2/H-1 NMR, which can apply a static load up to 0.75 MPa to the cartilage sample with 1.0 cm in diameter.3) Visualization of collagen structure in the articular cartilage by H-2/H-1 DQF CSI : In the intact bovine articular cartilage, we can depict the collagen structures in the superficial, tangenital, deep and carcified zones, Observation and analysis on the deformation of collagen structures under static loading are under going, and might be finished in 2001.