c-Src Signaling that regulates Cell Adhesion

本研究的目的是阐明c-Src调控细胞粘附的关键信号通路，该通路依赖于c-Src调控的可逆生化反应。本研究在我们以前报道Src激酶可以调节细胞粘附的基础上，进一步阐明细胞粘附依赖于细胞周期和NO信号的调节，发现Crk是一种能与c-Src结合激活c-Src的过继分子，通过Ras-MEK途径抑制cadherin依赖的细胞粘附。另外，我们发现e-NOS产生的NO可以直接激活c-Src激酶。

The aim of this study is to clarify the critical signaling pathway for the regulation of cell adhesion that depends on reversible biochemical reaction regulated by c-Src. Based on our previous report that Src kinase can regulate cell adhesion, we planned to clarify the regulation of cell adhesion dependent on cell cycle and NO-signaling.In this study we found that Crk, an adopter molecule that binds c-Src to activate it, suppressed cadherin-dependent cell adhesion via Ras-MEK pathway. In addition we found NO produced by e-NOS could directly activate c-Src kinase.