Tyrosine phosphrylating protein critical for cell-cell adhesion

酪氨酸磷酸化蛋白对细胞间粘附至关重要

• 批准号: 03670139
• 负责人: HAMAGUCHI Michinari
• 金额: $ 1.28万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670139/
• 关键词: src; tyrosine phosphorylation; cadherin; catenin; cytoskeleton; ハービマイシン; PI3′キナーゼ; がん化

Transformation of chick embryonic fibroblasts with Rous sarcoma virus strongly suppresses N-cadherin-mediated cell-cell adhesion, without inhibiting its expression. This suppression is correlated with tyrosine phosphorylation of N-cadherin and catenins, the cadherin-associated proteins, which are know to regulate cadherin function. Experiments with non-myristylation and temperature-sensitive mutants of RSV and with herbimycin A, a potent inhibitor of tyrosine kinases, suggest that both the suppression of cell adhesion and tyrosine phosphorylation of catenins are highly transformation-specific.

劳斯肉瘤病毒转化鸡胚成纤维细胞可明显抑制n-钙粘蛋白介导的细胞粘附，但不抑制其表达。这种抑制与n-钙粘蛋白和catenins的酪氨酸磷酸化有关，catenins是钙粘蛋白相关蛋白，已知可以调节钙粘蛋白的功能。对非肉豆荚化和温度敏感的RSV突变体以及有效的酪氨酸激酶抑制剂herbyycin A的实验表明，抑制细胞粘附和酪氨酸磷酸化连环蛋白都具有高度的转化特异性。

项目成果

M.Hamaguchi: "p60v-src causes tyrosine phosphorylation and inactivation of the N-cadherin-catenin cell adhesion system" EMBO J.12. 307-314 (1993)

M.Hamaguchi：“p60v-src 导致酪氨酸磷酸化和 N-钙粘蛋白-连环蛋白细胞粘附系统失活”EMBO J.12。

K.Shiroki,M.Hamaguchi and S.Kawai.: "Highly efficient focus formation by Rous sarcoma virus on Adenovirus 12 E1A transformed rat 3Y1 cells." J.Virol.

K.Shiroki、M.Hamaguchi 和 S.Kawai.：“劳斯肉瘤病毒在腺病毒 12 E1A 转化的大鼠 3Y1 细胞上高效形成病灶。”

N.Matsuyoshi: "Cadherin-mediated cell-cell adhesion is perturbed by v-src tyrosine phosphorylation in metastatic fibroblasts" J.Cell Biol.118. 703-714 (1992)

N.Matsuyoshi：“钙粘蛋白介导的细胞间粘附受到转移性成纤维细胞中 v-src 酪氨酸磷酸化的干扰”J.Cell Biol.118。

M.Hamaguchi: "Herbimycin A inhibits the association of p60v-src with cytoskeletal structure and with phosphatidylinositol3′kinase" Oncogene. 8. 559-564 (1993)

M. Hamaguchi：“Herbimycin A 抑制 p60v-src 与细胞骨架结构和磷脂酰肌醇 3 激酶的关联”Oncogene，8. 559-564 (1993)。

I.Nakashima et al.: "Evidence of synergy between Thyー1 and CD3/TCR comples in signal delivery to murine thymocytes for cell death." J.Immunol.147. 1153-1162 (1991)

I.Nakashima 等人：“Thy-1 和 CD3/TCR 复合物在向小鼠胸腺细胞传递细胞死亡信号方面的协同作用的证据。”J.Immunol.1153-1162 (1991)。