Basic ultrastructural and immunohistochemical studies for the structure and function of stromal cells: especially upon the unique anatomical structural unit of the "neuro-reticular complex"

基质细胞结构和功能的基础超微结构和免疫组织化学研究：特别是“神经网状复合体”独特的解剖结构单元

基本信息

批准号：
10670174
负责人：
YAMAZAKI Kazuto
金额：
$ 1.92万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

In stromal tissue of normal or physiological conditions, the existence of the unique anatomical unit of the "neuro-reticular complex", which consisted by reticular stromal cell network connected by gap junctions and terminated by autonomic nerve endings, was studied in variety of human stromal tissue, such as, fallopian tube mucosa, mammary gland, testis, and gallbladder mucosa etc.. The existence was confirmed not only in hematopoietic tissues (bone-bone marrow, spleen, lymph nodes) but submandibular gland, thyroid gland and fallopian tub mucosa. Characterization of the stromal fibroblasts was also attempted in the tissues. The distribution of connexins, mainly of connexin 26, --constitutional proteins of gap junction, was studied in melanocytes of human vestibular inner ear. The differentiation of osteoclasts was studied in primitive bone marrow tissue of C3H fetal mice with the development of gap junctions.Pathological human stromal tissues of epiretinal membrane of highly myopic eyes and lacrimal gland of cGVHD patients suffered dry eyes were studied, in which gap junctions and CD34 positive stromal fibroblasts were assumed to play significant roles. In stromal tissues of malignant tumors and giant cell fibroblastoma, hitherto-not-well-described and unique basal lamina like amorphous extracellular materials were newly found.The methodological establishment of the in situ hybridization using probes of virus genomes was succeeded to expect future applications of stromal tissue studies.Characterization of stromal fibroblasts, distribution of the neuro-reticular complex, and CD 34 positive reticular networks etc., were contributed to the comprehensive undeistandings of stromal tissues.

In stromal tissue of normal or physiological conditions, the existence of the unique anatomical unit of the "neuro-reticular complex", which consisted by reticular stromal cell network connected by gap junctions and terminated by autonomic nerve endings, was studied in variety of human stromal tissue, such as, fallopian tube mucosa, mammary gland, testis, and gallbladder mucosa etc.. The不仅在造血组织（骨骨骨髓，脾，淋巴结）中证实了存在，还证实了下颌下腺，甲状腺腺体和输卵管肿大的粘膜。在组织中还尝试了基质成纤维细胞的表征。在人前庭内耳的黑素细胞中研究了连接蛋白的分布，主要是连接26的 - 固定蛋白。在C3H胎儿小鼠的原始骨髓组织中研究了破骨细胞的分化。与高度近视眼睛的皮肤膜的人类基质组织的高度近视眼和CGVHD患者的泪腺呈干眼睛的眼睛，并在其中遭受了差异的差异，并在gap naked sunder flans中均为差异。在恶性肿瘤和巨大细胞成纤维细胞瘤的间质组织中，新发现的迄今未描述和独特的基础层（如无定形细胞外材料）是新发现的。方法论在原位杂交建立了病毒基因组探针的原位杂交，预计未来的拟层型纤维应用。神经性视网膜复合物和CD 34阳性网络网络等有助于基于基质组织的全面不稳定。