Genetic and biochemical analysis of insecticide-insensitivity of acetylcholinesterase in vector mosquitoes

媒介蚊乙酰胆碱酯酶对杀虫剂不敏感的遗传和生化分析

• 批准号: 10670246
• 负责人: TOMITA Takashi
• 金额: $ 2.05万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670246/
• 关键词: acetylcholinesterase; Culex tritaeniorhynchus; insecticide resistance; organophosphate; linkage analysis; cDNA; protein polymorphism; 有機リン剤

Protein polymorphism attributable to organophosphorus(OP) insecticide-insensitivity of acetylcholinesterase(AChE) was investigated with mosquitoes of three insecticide-suceptible strains and the OP-resistant strain, Toyama, and mosquitoes newly collected from the natural population in Yokohama of Culex tritaeniorhyncus (C.t.). All the 20 field mosquitoes tested contained OP-insensitive AChE as well as Toyama strain. No protein sequence polymorphism was detected in the deduced matured peptide of AChE in all the tested mosquitoes.A comparative linkage map for the mosquito, Culex tritaeniorhynchus, was constructed based on restriction fragment length polymorphism (RFLP) markers using cDNA clones from Aedes aegypti and AChE cDNA from C.t. OP resistance is controlled by a major gene (AChE^R) on chromosome 2, while the AChE structural gene (Ace) is located on chromosome 1. No evidence for a second Ace gene was observed, suggesting that the AChE^R locus represents a modifier of insecticide-sensitivity of AChE.

以三种杀虫剂敏感品系和 OP 抗性品系 Toyama 蚊子以及从横滨自然种群中新采集的三带库蚊 (C.t.) 蚊子为对象，研究了有机磷 (OP) 乙酰胆碱酯酶 (AChE) 对杀虫剂不敏感的蛋白质多态性。所有 20 只测试的野外蚊子都含有 OP 不敏感的 AChE 以及 Toyama 菌株。所有测试的蚊子的AChE成熟肽均未检测到蛋白质序列多态性。利用埃及伊蚊的cDNA克隆和三带喙库蚊的AChE cDNA，基于限制性片段长度多态性（RFLP）标记构建了三带喙库蚊的比较连锁图谱。 OP 抗性由 2 号染色体上的主基因 (AChE^R) 控制，而 AChE 结构基因 (Ace) 位于 1 号染色体上。没有观察到第二个 Ace 基因的证据，表明 AChE^R 位点代表 AChE 杀虫剂敏感性的修饰因子。

项目成果

Mori A,Tomita T, Hidoh O,Kono Y,Severson DW: "Comparative linkage map development and identification of an autosomal locus for insensitive acetyl cholinesterase-mediated insecticide resistance in culex tritaeniorhyncus."Insect Molecular Biology. 10(2)(in

Mori A、Tomita T、Hidoh O、Kono Y、Severson DW：“三带库蚊中不敏感乙酰胆碱酯酶介导的杀虫剂抗性的常染色体基因座的比较连锁图谱开发和鉴定。”昆虫分子生物学。