Genetic study on overexpression mechanisms of cytochrome P450 in insecticide resistant insect

抗药性昆虫细胞色素P450过表达机制的遗传学研究

• 批准号: 19580062
• 负责人: TOMITA Takashi
• 金额: $ 2.91万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19580062/
• 关键词: 殺虫剤抵抗性; シトクロムP450; 遺伝子過剰発現; 遺伝子重複; シス作用性調節変異; ネッタイイエカ; ピレスロイド; シトクロムP450モノオキゲナーゼ; DNA増幅; 殺虫剤解毒機構; アカイエカ種群蚊; シトウロムP450モノオキゲナーゼ

A P450 gene Cyp9m10 is about 260-fold overexpressing in a pyrethroid-resistant strain of Culex quinquefasciatus compared to a susceptible strain. a region of approximately 100kb in length including the Cyp9m10 locus was specifically duplicated in the resistant strain. The two duplicated Cyp9m10 copies shared a completely identical sequence within the transcribed region and the flanking region up to the breakpoint located 1.1kb upstream of the transcriptional start site. A Miniature Inverted-repeat Transposable Element (MITE)-like element was specifically inserted 0.2kb upstream of both Cyp9m10 copies in the resistant strain. We obtained direct evidence that the Cyp9m10 overexpression is caused by a cis-acting mutation. In backcross experiment, a haplotype containing the two duplicated Cyp9m10 copies was strongly associated with the pyrethroid resistance.

P450基因Cyp9m10在致倦库蚊拟除虫菊酯抗性品系中的过表达是敏感品系的260倍。包括Cyp9m10基因座在内的长约100kb的区域在抗性菌株中特异性复制。两个复制的Cyp9m10拷贝在转录起始位点上游1.1kb的断裂点处的转录区和侧翼区具有完全相同的序列。在该抗性菌株的Cyp9m10基因上游0.2kb处特异性插入了一个MITE样元件。我们获得的直接证据表明Cyp9m10过表达是由顺式作用突变引起的。在回交试验中，Cyp9m10的单倍型与拟除虫菊酯抗性密切相关。

项目成果

Insecticide resistance in the mosquito Culex pipiens complex

淡色库蚊复合体的杀虫剂抗性

• 发表时间: 2008
• 作者: Takashi Tomita;(他2名)
• 通讯作者: (他2名)

Overexpression of cytochrome P450 genes in pyrethroid-resistant Culex quinquefasciatus

• DOI: 10.1016/j.ibmb.2010.01.006
• 发表时间: 2010-02-01
• 期刊: INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
• 影响因子: 3.8
• 作者: Komagata, Osamu;Kasai, Shinji;Tomita, Takashi
• 通讯作者: Tomita, Takashi

アカイエカ種群蚊の殺虫剤抵抗性及び亜種判別に関わる遺伝子型の個体別決定法

库蚊抗药性个体基因型测定方法及亚种区分

• 发表时间: 2007
• 作者: 駒形修;(他6名)
• 通讯作者: (他6名)

Advances in Human Vector Control.(Clark, J. M., Bloomquist, J. R., Kawada, H. (Eds.), Insecticide resistance in the mosquito Culex pipiens Complex)

人类病媒控制进展。（Clark, J. M.、Bloomquist, J. R.、Kawada, H.（编辑），蚊子库蚊复合体的杀虫剂抗性）

• 发表时间: 2009
• 作者: Osamu Komagata;Shinji Kasai;Takashi Tomita
• 通讯作者: Takashi Tomita

殺虫剤抵抗性ネッタイイエカで高発現しているピレスロイド代謝性P450遺伝子の発現機構に関する研究

抗药库蚊高表达拟除虫菊酯代谢P450基因表达机制研究

• 发表时间: 2009
• 作者: 糸川健太郎;(他5名)
• 通讯作者: (他5名)