Large expression and application of the measles virus neutralization antigen H and F protein using the virus engineering technique.

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2 kinds of recombination baculoviruses that manifested the H protein were produced in order to carry out three-dimensional structure analysis of the important hemagglutinin protein (H protein) as a protective antigen of measles virus. Except for cytoplasm domain of the H gene, recombination baculovirus which connected the HA protein signal sequence of the influenza virus A/Japan strain (H3 subtype) was produced so that the H protein may secrete it to culture medium from the infected insect cells. The second viruses produced recombinant baculovirus which introduced cutting arrangement of factor-Xa into cell membrane attachment protein of the H protein. The protein was refined by affinity chromatography, ion exchangechromatography and gel filtration chromatography using specific antibody in order to large recover these 2 kinds of H proteins. With the goof the stage of the purification, the reactivity with the monoclonal antibody for measles virus lowered through the H protein. That H pro … More tein which reacts with the monoclonal antibody which shows only neutralizing antibody existed only for the fractionation in which electrophoresis and gel filtration chromatography recognize the large aggregation clarified. And, the activity which also hemagglutination of the green monkey red blood cells in either protein could not be recognized. The isolated purification of the protein from the recombinant baculovirus for structure analysis seems to be unsuitable from the above result. Therefore, it is concluded that the isolated purification of the protein from the virion is important in order to carry out three-dimensional structure analysis of the virus.And, we had already sensitized the measles virus in the gelatin particle, and the new measles antibody measuring methodwas developed. This was applied this time, and the measles IgM antibody measuring method was established. It was adopted for the measles eradication plan of WHO, because this method is convenient, and it would be examined in developing countries in Africa and South America, etc. Less