DNA typing without extracting DNA.

DNA提取试剂盒大量上市，样品的准备变得相当容易。然而，如果是在一般使用的方法中直接放入样品来完成的，则是最简单的。这次对不提取DNA直接进行聚合酶链式反应的方法进行了检验，采血后的血液没有被聚合酶链式反应扩增，因此DNA固定在细胞上，不起模板的作用。然而，当它经过2，3天时，可以作为足够的样品使用。在不提取DNA的情况下，成功地对血清、全血、唾液、指甲、血痕、尿液和胡须等样品进行了STR分型，该方法简单、快速，将在法医实践中得到应用。

The extraction kit of DNA is marketed in great numbers, and the preparation of the sample becomes considerably easy. However, the PCR is the simplest, if it is completed by putting direct sample in generally using method. This time, the method that it did not extract DNA and does the direct PCR was examined.The blood right after blood collection was not amplified by the PCR.As this cause, DNA was fixed to cell, and it did not work as a template. However, it was possible to use as sufficiently sample, when it passes through the 2,3 day. STR typing was successfully applied to various samples such as serum, whole blood, saliva, nail, blood stain, urine and mustache without extracting DNA from them.The simple and fast methods for PCR as demonstrated herein will be useful in the field of forensic practice.