DNA typing without extracting DNA.

无需提取 DNA 即可进行 DNA 分型。

• 批准号: 10670381
• 负责人: UMETSU Kazuo
• 金额: $ 2.43万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670381/
• 关键词: DNA typing; PCR; STR; DNA増幅; PCR法; DNA多型; STR多型; 直接検出法; AmpliTaq Gold; 血液

The extraction kit of DNA is marketed in great numbers, and the preparation of the sample becomes considerably easy. However, the PCR is the simplest, if it is completed by putting direct sample in generally using method. This time, the method that it did not extract DNA and does the direct PCR was examined.The blood right after blood collection was not amplified by the PCR.As this cause, DNA was fixed to cell, and it did not work as a template. However, it was possible to use as sufficiently sample, when it passes through the 2,3 day. STR typing was successfully applied to various samples such as serum, whole blood, saliva, nail, blood stain, urine and mustache without extracting DNA from them.The simple and fast methods for PCR as demonstrated herein will be useful in the field of forensic practice.

DNA提取试剂盒大量上市，样品的制备变得相当容易。而PCR法是最简单的方法，一般采用直接进样的方法。这次检查了不提取DNA直接进行PCR的方法。采血后的血液没有被PCR扩增。由于这个原因，DNA被固定在细胞上，它不能作为模板。然而，当它经过2，3天时，可以用作足够的样品。STR分型方法成功地应用于血清、全血、唾液、指甲、血迹、尿液和胡子等样本的STR分型，不需要提取DNA，方法简便、快速，在法医学领域具有重要的应用价值。

项目成果

梅津和夫: "DNA非抽出法による多形解析"DNA多型. 8. (2000)

Kazuo Umezu：“使用非DNA提取方法的多态性分析”DNA多态性8。（2000）。

梅津和夫: "DNA多型、Vol8"東京東洋書店. 3 (2000)

梅津和夫：“DNA 多态性，第 8 卷”东京东洋书店 3（2000 年）。

Kazuo Umetsu: "DNA typing without extracting DNA"DNA polymorphism, (To yo Shoten). Vol.8. 17-19 (2000)

Kazuo Umetsu：“不提取DNA的DNA分型”DNA多态性，（To yo Shoten）。

梅津和夫: "DNA多型"東洋書店. 3 (2000)

梅津和夫：《DNA 多态性》东洋书店 3 (2000)。