Establishment of personal identification method using a simple fluorescence APLP method for SNP testing

共选择了47个在欧洲、中国、日本、非洲人群中高度多态的SNPs（HapMap数据库）。我们开发了一种可靠、快速、经济的多重扩增产物长度多态性（APLP）方法来分析47个SNP。我们可以安全地使用四个12-多重PCR和随后的10%聚丙烯酰胺凝胶电泳进行基因分型。扩增子的长度范围为40至100 bp。在所研究的所有群体中，平均匹配概率约为5 × 10 ~ 4<-20>。我们的APLP检测是一个只有两个步骤的过程，只需要多重扩增反应和天然聚丙烯酰胺凝胶电泳上的substrate运行。

A total of 47 SNPs reported to be highly polymorphic in European, Chinese, Japanese, African populations (HapMap database) were selected. We developed a reliable, rapid, and economical multiplex amplified product-length polymorphism (APLP) method for analyzing the 47 SNPs. We could safely be genotyping by using four 12-multiplex PCR and subsequent 10% polyacrylamide gel electrophoresis. The amplicons ranged from 40 to 100 bp in length. The mean match probability was about 5 X 10^<-20> in all populations studied. Our APLP assay is an only two-step process that requires just mulitiplex amplification reaction and asubsequent run on native polyacrylamide gel electrophoresis.