The Biological Functions of the SART-1 Family in Differentiation and Carcinogenicity of Adenocarcinomas

Molecules involved in gulation of cellular proliferation at the G2/M phase not been fullyidentified. This study investigated the involvement of a recently identified SART-1 D2800 - D2.Protein demonstrated an ability to bind to DNA，and accumulated in the nucleus when the cells were arrested at G2/M phase.(l) the localization ofSART-1 protein was suggested to be in nucleus of eukaryote cells including monkey kidneyEBV-transformed Cos 7 cell and human esophageal cancer TE9 cell when the fusion gene with GFP wastransfected and observed by conforcal laser microscopy.(2) SART-1 - D2800 - D2 protein is suggested tobe a DNA-binding protein by the affinity chromatography of the 293T cellstransfected with the HAN/SART-1 D2800 - D2 gene.(3) Transfection of SART-1 gene induced theG2/M-arrest and suppressed the cell growth of all the cell tested. Furthermore，DNA ladder formation was observed in the sat -1 transfected COS-7 and human cancer cells. thetransfection of SART-1 up-regulated the nuclear expression of cyclin B和cdc2，良好的已知组件of maturation-promoting factor.(4) Several candidate genes which encodeproteins that had interacted with SART-1 protein were isolated by two-hybrid system.(5) Threedifferent sat -1 probe protein （N-terminal, N-terminal）middle and C-terminal regions for farr -western blot analysis of SART-1 binding protein candidate(6)我们发现了murine SART-1 genomic DNA suggested tobe consist of 20 exsons. The sequence of The gene will submit to GenBank.(7) SART-1 gene wassuggested to be expressed in early development of mouse embryo (l4 days) by northern blotSeveral family genes of SART-1 were suggested by northern blot analysis. One of thegene with 3.3kb consist of approximately l488 bp of 5'-region was identical to SART-1 and encoded aprotein with 483 amino acid。

Molecules involved in regulation of cellular proliferation at the G2/M phase have not yet been fully identified. This study investigated the involvement of a recently identified SART-1ィイD2800ィエD2. Protein demonstrated an ability to bind to DNA, and accumulated in the nucleus when the cells were arrested at G2/M phase.(l) The localization of SART-1 protein was suggested to be in nucleus of eukaryote cells including monkey kidney EBV-transformed Cos 7 cell and human esophageal cancer TE9 cell when the fusion gene with GFP was transfected and observed by conforcal laser microscopy.(2) SART-1ィイD2800ィエD2 protein is suggested to be a DNA-binding protein by the affinity chromatography of cell lysates of the 293T cells transfected with the HAN/SART-1ィイD2800ィエD2 gene.(3) Transfection of SART-1 gene induced the G2/M-arrest and suppressed the cell growth of all the cell tested. Furthermore, DNA ladder formation was observed in the SART-1 transfected COS-7 and human cancer cells. The transfection of SART-1 up-regulated the nuclear expression of cyclin B and cdc2, well known components of maturation-promoting factor.(4) Several candidate genes which encode proteins that had interacted with SART-1 protein were isolated by two-hybrid system.(5) Three different SART-1 probe protein (N-terminal, middle and C-terminal regions) for far-western blot analysis of SART-1 binding protein candidate obtained by two hybrid system were prepared.(6) We found that murine SART-1 genomic DNA suggested to be consist of 20 exsons. The sequence of the gene will submit to GenBank.(7) SART-1 gene was suggested to be expressed in early development of mouse embryo (l4 days) by northern blot analysis.(8) Several family genes of SART-1 were suggested by northern blot analysis. One of the gene with 3.3kb consist of approximately l488 bp of 5'-region was identical to SART-1 and encoded a protein with 483 amino acid.