Detection of mRNAscharacteristically expressed in heart failure transiti

利用达尔盐敏感大鼠，我们最近建立了一种动物模型，以可重复的方式观察到从机械代偿的同心型左室肥大到其失代偿伴左室功能障碍和扩大的转变。在本研究中，我们通过差异显示RT-PCR （DD）方法，探讨了代偿性LVH和LV衰竭阶段心肌mRNA表达的明显差异。通过DD比较，我们检测到130个mRNA片段，并成功进行了TA克隆。随后的同源性搜索显示，它们由23个已知基因和14个未知基因组成。在已知的基因组中，发现了编码心房利钠肽和α -心脏MHC等mrna。有趣的是，有几种mrna的编码因子被认为与心力衰竭或心脏重构无关。因此，我们将阐明这些mrna编码的因子在心力衰竭中的病理生理作用。

Using Dahl salt-sensitive rats, we recently developed an animal model in which transition from mechanically compensated concentric LV hypertrophy to its decompensation with LV dysfunction and enlargement was observed in a reproducible manner. In the present study, we explored myocardial mRNA expressions that showed distinct difference between the stages of compensated LVH and LV failure, by means of differential display RT-PCR (DD) methods. We detected 130 mRNA fragments along with the DD comparison and these mRNAs were successfully TA cloned. The subsequent homology search reveled that they consisted of 23 know genes and 14 unknown genes. In the known gene group, mRNAs coding such as atrial natriuretic peptide and alpha-cardiac MHC were found. Interestingly, there were several mRNAs that code factors having been assumed to be independent of heart failure or cardiac remodeling. Thus, we are going to elucidate the pathophysiological roles of these factors coded by these mRNAs on the heart failure.