Investigation of serotonergic regulation of acetycholine release in the cortex (II)

（Exp。1） The role of 5-HTイイD21AイエD2 receptors in the serotonergic regulation of cortically projecting cholinergic neurons was studed。8-OH-DPAT（DPAT ; 1.0毫克/千克，s. C）显著增加的Cortical ACh输出。WAY 100-635 （WAY，0.1毫克/千克，秒）这并不影响ACh的输出。WAY的本地应用（0.1， 10μM，60分钟）在前端Cortex对基本ACh输出没有影响。注射苯氟胺后局部应用途径（10微米，60分钟）（FEN ; 10毫克/千克，一页）DOI （2.5毫克/千克）一、P）这并不影响ACh的输出。Nevertheless， DPAT （10 μM） applied after injection of DOI significantly potentiated DOI's ability to increase cortical ACH release。酮素后注射DPAT（KET ; 5毫克/千克，包括）未能影响到ACh的输出。这些结果提出了三个可能的问题。第一，5-HT可能不是今晚刺激ACh释放通过后synaptic 5-HT-E-第二，FEN-诱导的ACh释放可能主要通过5-HT-D22 A-D2接收而被介导。 ... More ors。Third， the stimulatory effect of 5-HT on ACh release via the postsynaptic 5-HT-D21A-D2 receptors may be increased when the 5-HT-D22A-D2 receptors are activated。（Exp。（2）“雌激素对医学预测的慢性神经元的影响”（2）。九天后，异位瘤大鼠植入了微透析探针，并直接注射了每17β-雌二醇（3.0微克/千克）。30 g/kg、300 g/kg），或sesame oil。两天后的微透析实验已经完成。17β-雌二醇治疗结果在每个个体动物组中的血清雌二醇浓度中的显著差异。然而，它失败了，无法在基本ACh输出中产生不同。在补充剂中，利用替代剂并没有在FEN的能力中产生差异，从而增加了Cortech释放。这些结果建议说，两天的雌激素替换是不够的，要恢复两者之间的功能的cortically projecting cholinergic neurons，以及5-HT D22 A Eye D2 receptors。Less（低）

〔Exp. 1〕 The role of 5-HTィイD21AィエD2 receptors in the serotonergic regulation of cortically projecting cholinergic neurons was studied. 8-OH-DPAT (DPAT ; 1.0 mg/kg, s.c.) significantly increased cortical ACh output. WAY 100-635 (WAY ; 0.1 mg/kg, s.c.) did not influence the ACh output. Local application of WAY (0.1, 10μM for 60 min) in the frontal cortex had no effect on the basal ACh output. Locally applied WAY (10 μM for 60 min) after injection of fenfluramine (FEN ; 10 mg/kg, i.p.) or DOI (2.5 mg/kg. i.p.) did not affect the ACh output. Nevertheless, DPAT (10 μM) applied after injection of DOI significantly potentiated DOI's ability to increase cortical ACh release. DPAT injected after ketanserin (KET ; 5 mg/kg, i.p.) failed to affect the ACh output. These results suggest three possibilities. First, 5-HT may not tonically stimulate ACh release via the postsynaptic 5-HTィイD21AィエD2 receptors. Second, FEN-induced increase of ACh release may be mediated mainly via the 5-HTィイD22AィエD2 recept … More ors. Third, the stimulatory effect of 5-HT on ACh release via the postsynaptic 5-HTィイD21AィエD2 receptors may be increased when the 5-HTィイD22AィエD2 receptors are activated.〔Exp. 2〕 The effects of estrogen on cortically projecting cholinergic neurons were investigated. Nineteen days after ovariectomy rats were implanted a microdialysis probe, and immediately injected subcutaneously with either 17β-estradiol (3.0μg/kg. 30μg/kg, 300μg/kg), or sesame oil. Two days later microdialysis experiment was performed. 17β-estradiol treatment resulted in a significant difference among the serum estradiol concentrations of each individual animal group. However, it failed to produce a difference in the basal ACh output. In addition, estrogen replacement did not yield a difference in FEN's ability to increase cortical ACh release. These results suggest that estrogen replacement for two days is not enough to restore both the functions of cortically projecting cholinergic neurons, and of 5-HTィイD22AィエD2 receptors. Less