Effects on neuronal formation of the hippocampus in developmental abnormalities

对发育异常中海马神经元形成的影响

• 批准号: 10670904
• 负责人: TAKEUCHI Yoshiki
• 金额: $ 2.43万
• 依托单位: Kagawa Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670904/
• 关键词: Developmental abnormality; Hippocampus; Prenatal X-irradiation; Ethanol; Neuronal formation; Granule cells; Pyramidal cells; Glia cells; 胎児性アルコール症候群; 海馬錐体細胞; 学習・行動解析; 遅発性神経細胞死; 細胞障害メカニズム; 発育・発達遅延; 行動異常; アルコール; PND16; PND30; 組織定量; ニューロン発

The effects of prenatal X - irradiation and ethanol exposure on neuronal development of the hippocampus were investigated in the present study. At six weeks of age in the rats the laminal structure of the hippocampus was deranged with exposure of 0.6 Gy or more. Pyramidal cells in the CA3 region were more susceptible than those in the CA1. Abberant mossy fiber terminals were observed in the stratum onence of the CA3 region with exposure of 0.3 Gy or more.On the other hand, exposing neonate rats to high dose of ethanol affected Purkinje cell numbers in the cerebellum. The period between 4 and 9 day of age was an extremely vulnerable period during which the Purkinje cells were particularly susceptible to the effects of a high dose of ethanol. However, a similar level and duration of ethanol exposure commencing after 10 day of age had no significant effect on the cell numbers. In contrast to granule and pyramidal cells numbers, neurons in the hilus region were indicated to be particularly vulnerable to the effects of a high dose of ethanol exposure during early postnatal life. In addition, short-term ethanol exposure to mice led to a decrease in the total number of calbindin-IR neurons and an increase in GFAP-IR glia cells. The disruption of calbundin and GFAP could affect neuronal - astroghal interaction, resulting in disturbance of behaviors dependent on the hippocampus.

在本研究中研究了产前X-辐照和乙醇暴露对海马神经元发育的影响。在大鼠的六周龄时，海马的层状结构被污染，暴露于0.6 Gy或更多。 CA3区域的锥体细胞比CA1中的锥体细胞更易感。在CA3区域的层次上观察到宽大的苔藓纤维末端，另一方面暴露于0.3 Gy或更多。 4至9天之间的周期是一个极其脆弱的时期，在此期间，purkinje细胞特别容易受到高剂量乙醇的影响。但是，在10天之后开始的乙醇暴露水平和持续时间对细胞数没有显着影响。与颗粒和锥体细胞数量相反，hilus区域的神经元被认为特别容易受到产后早期早期乙醇暴露的影响。此外，短期乙醇暴露于小鼠导致Calbindin-IR神经元数量减少和GFAP-IR胶质细胞的增加。钙蛋白钙蛋白酶和GFAP的破坏可能影响神经元 - 星形镜的相互作用，从而导致行为的干扰，取决于海马。

项目成果

Miki T: "A stereological analysis of the effect of early postnatal ethanol exposure on neuronal numbers in rat dentate gyrus."Image Analysis and Stereology. 19. 99-104 (2000)

Miki T：“产后早期乙醇暴露对大鼠齿状回神经元数量影响的体视学分析。”图像分析和体视学。