Development of BRM sensitivity test using the Th1/Th2 balance and clinical application

在这项研究中，我们评估了外周血淋巴细胞（pbl） Th1/Th2平衡对使用OK-432进行癌症免疫治疗监测的有效性。以56例治愈性胃癌患者为研究对象，其中OK-432给药组38例，非给药组18例，OK-432给药组3例，每2周皮内给药1次。与未给药组相比，给药组PBLs中IFN- γ和IL-2的生成量显著增加，而IL-4的生成量无显著差异。根据术后3个月NK活性将给予OK-432的患者分为高反应组和低反应组。在使用OK-432的患者中，Th1/Th2的平衡改变为Th1优势状态。OK-432高反应患者与低反应患者相比，Th1/Th2比值明显升高，证实了Th1优势的免疫状态。这些结果表明，PBLs的Th1/Th2平衡是监测胃癌患者使用OK-432免疫治疗的有用指标。

In this study, we assessed the usefulness of Th1/Th2 balance of peripheral blood lymphocytes (PBLs) for therapeutic monitoring of cancer immunotherapy using OK-432. A Total of 56 curative gastric cancer patients (OK-432 administration group : 38 patients ; non-administration group : 18 patients) were used as subjects, and 3 KE of OK-432 was administered intradermally once every 2 weeks for OK-432 administration group. The production of IFN- γ and IL-2 of PBLs in the OK-432-administered group were significantly higher than that of OK-432-non administered group, while the production of IL-4 was not significantly different between them. The patients who had been administered OK-432 were divided into two groups (high-response and low-response) according to the NK activity at three months after surgery. In patients administered OK-432, the balance of Th1/Th2 was changed to Th1-dominant status. OK-432 high response patients showed a significant increase in the ratio of Th1/Th2, as compared to low response patients, and Th1-dominant immunological status was confirmed.These results suggest that the Th1/Th2 balance of PBLs is a useful indicator for monitoring of immunotherapy using OK-432 in patients with gastric cancer.