MECHANISMS OF THE NERVE PLEXUS INVASION BY GASTROINTESTINAL CANCERS

胃肠道癌侵袭神经丛的机制

• 批准号: 10671179
• 负责人: DOI Ryuichiro
• 金额: $ 2.05万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671179/
• 关键词: gastrointestinal cancer; invasion; pancreatic cancer; 消火器癌; 浸潤; 消化器 癌; 神経浸潤; 手術

A migration assay with Boyden chamber and a growth assay were performed on pancreatic cancer cell lines, AsPC-1 and BxPC-3. The effects of nerve growth factor (NGF) on the pancreatic cancer cell migration and cell growth were evaluated. NGF showed no significant effects on pancreatic cancer cell migration and cell growth. PC-12 cell derived from pheochromocytoma is known to possess a character of nerve cells. After PC-12 cells differentiated to nerve-like cells, they were set in the lower chamber of the Boyden chamber. A migration assay for pancreatic cancer cells were performed. The addition of differentiated PC-cells in the lower chamber had no effects on the pancreatic cancer cell migration. NCAM is expressed in the nerve cells. The expression of NCAM in the pancreatic cancer tissue was examined immunohistochemically. Approximately 60% of 40 cases of pancreatic cancer expressed NCAM. Western blot and RT-PCR examined thd expressions of NCAM in pancreatic cancer cell lines, and a number of pancreatic cancer cells expressed NCAM. Intraoperative rapid immunohistochemistry for cytokelatin-19 in nerve plexus were tested. The micrometastases in the plexus were detected by this method when HE stain results negative. This method might give an information if additional radiation therapy is necessary after surgical resection of the pancreatic cancer.

对胰腺癌细胞系AsPC-1和BxPC-3进行Boyden室迁移测定和生长测定。评价神经生长因子（NGF）对胰腺癌细胞迁移和细胞生长的影响。NGF对胰腺癌细胞的迁移和生长无明显影响。已知来源于嗜铬细胞瘤的PC-12细胞具有神经细胞的特征。PC-12细胞分化为神经样细胞后，将其置于博伊登室的下室中。进行胰腺癌细胞的迁移测定。在下腔室中加入分化的PC细胞对胰腺癌细胞迁移没有影响。NCAM在神经细胞中表达。免疫组化法检测胰腺癌组织中NCAM的表达。40例胰腺癌中约60%表达NCAM。Western blot和RT-PCR检测胰腺癌细胞系中NCAM的表达，大量胰腺癌细胞表达NCAM。术中行神经丛细胞角蛋白19免疫组化快速检测。当HE染色阴性时，用此方法检测丛内的微转移。这种方法可能会提供一个信息，如果额外的放射治疗是必要的手术切除后的胰腺癌。

项目成果

Koshiba, T., Doi, R.: "Immunohistochemical analysis of cyclooxygenase-2 expression in pancreatic tumors."International Journal of Pancreatology. 26. 69-76 (1999)

Koshiba, T., Doi, R.：“胰腺肿瘤中环氧合酶 2 表达的免疫组织化学分析。”国际胰腺学杂志。

Kobayashi,H.,Doi,R.: "Immunohistochemical analysis of apoptosis-related proteins in human embryonic and fetal pancreatic tissues"International Journal of Pancreatology. (印刷中).

Kobayashi, H., Doi, R.：“人类胚胎和胎儿胰腺组织中凋亡相关蛋白的免疫组织化学分析”《国际胰腺学杂志》（正在出版）。

Fujimoto,K.,Doi,R.: "Expression of two angiogenic factors,vascular endothelial growth factor arid platelet-derived endothelial cell growth factor in human pancreadic cancer" European Journal of Cancer. 34・9. 1439-1447 (1998)

Fujimoto, K., Doi, R.：“两种血管生成因子、血管内皮生长因子和血小板源性内皮细胞生长因子在人类胰腺癌中的表达”欧洲癌症杂志 34・9 (1998)。

Miyamoto Y.Doi,R.: "Immunohistochemical analysis of Bcl-2,Bax,Bcl-X, and Mcl-1 expression in pancreatic cancers"Oncology. 56. 73-82 (1999)

Miyamoto Y.Doi,R.：“胰腺癌中 Bcl-2、Bax、Bcl-X 和 Mcl-1 表达的免疫组织化学分析”肿瘤学。

Koshiba,T.,Doi R.: "Involvement of matrix metalloproteinase-2 activity in invasion and metastasis of pancreatic carcinoma" Cancer. 82. 642-650 (1998)

Koshiba,T.,Doi R.：“基质金属蛋白酶 2 活性参与胰腺癌的侵袭和转移”癌症。