The study of the control mechanisms of gastric motility and the change after function preserving operation.

胃运动控制机制及功能保留术后变化的研究。

• 批准号: 10671190
• 负责人: OKAJIMA Masazumi
• 金额: $ 1.92万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671190/
• 关键词: gastrointestinal motility; autonomic nerve preserving surgery; strain gauge force transducer; barostat; function preserving surgery; nitrogen oxide; gastric adaptive relaxation; pylorus preserving pancreatoduodenectomy; Strain Gauge Force Transduc

The study was designed to analyze canine gastroduodenal motility using strain gauge force transducers (SGTs) or barostat, and to clarify the control mechanisms of the motility, especially the pylorus and the fundus.We established the method to evaluate the canine pyloric opening using the SGTs. The relaxation and opening of the pyloric ring was observed synchronously with intense construction of the antrum during the phase III of normal dogs. As the same manner, phase III-like motility, which was not a typical periodic motility, was observed in the - pylorus-preserving pancreatoduodenectomy (PPPD) dogs. The phase III-like motility was induced by LeuィイD113ィエD1-motilin in both normal and PPPD dogs. Therefore. LeuィイD113ィエD1-motilin must improved dysmotility of the pylorus after PPPD.We also clarified the role of cholinergic and nitrergic inputs in mechanically and chemically induced gastric relaxation in conscious dogs. When mechanical stimulation, was applied by insufflating the intragastric bag at constant rate, the recordings of barostat showed the steep linear increase in intragastric pressure to about 6 mmHg. After that, the pressure had continued to increase gradually. NィイD1GィエD1-monomethyl-L-arginine acetate (L-NMMA) increased the pressure/volume gradient, while atropine reduced it significantly. An oral intake of lipid as chemical stimulation rapidly increased the proximal gastric volume (150.0±50.2ml). Although the lipid-induced increase in gastric volume subsided after atropine i.v. (36.0±14.7 ml ; p<0.005), it was unaffected by treatment with L-NMMA (167.6±53.0ml). The study shows that nitrergic input is not necessary for chemically induced gastric relaxation, but it is necessary for mechanically induced one. On the other hand, continuous cholimergic input is necessary for the both.

本研究旨在利用应变力传感器(SGT)或恒压计分析犬的胃十二指肠运动，阐明其运动的控制机制，特别是幽门和胃底的运动控制机制。在正常犬的第III期，观察到幽门环的松弛和开放与胃窦的紧张构筑同步进行。同样，在保留幽门的胰腺十二指肠切除术(PPPD)犬中也观察到非典型的周期性运动的III相样运动。亮氨酸ィイD113、ィエD_1-胃动素对正常犬和产后抑郁犬均有类III时相运动的诱导作用。因此。亮氨酸ィイD113ィエD1-胃动素必须改善PPPD后幽门的运动障碍。我们还阐明了胆碱能和氮能输入在机械和化学诱导的清醒狗胃松弛中的作用。机械刺激恒速灌胃时，恒压记录显示胃内压直线上升，约为6 mm Hg。在那之后，压力继续逐渐增加。NィイD1GィエD1-单甲基-L-精氨酸乙酸酯(N-NMMA-NMMA)显著增加压力/容量梯度，而阿托品显著降低压力/容量梯度。口服脂质作为化学刺激，可迅速增加近端胃容量(150.0±50.2ml)。尽管阿托品静脉注射后脂质引起的胃容量增加消退。L治疗组(167.6±53.0ml)与对照组比较，差异无统计学意义(P>0.005)。研究表明，氮能输入对化学诱导的胃松弛不是必需的，但对机械诱导的胃松弛是必要的。另一方面，对两者来说，持续的胆碱能输入是必要的。

项目成果

K. Toyota: "Pyloric opening and closure evaluated by means of strain gauge force transducers"Journal of Smooth Muscle Research. 34. 13-22 (1998)

K. Toyota：“通过应变仪力传感器评估幽门的打开和关闭”平滑肌研究杂志。

吉川雅文: "胃弛緩運動におけるコリン作動性機構の関与"Therapeutic Research. 20. 2334-2335 (1999)

Masafumi Yoshikawa：“胃松弛中胆碱能机制的参与”治疗研究 20. 2334-2335 (1999)。

Pyloric opening and closure evaluated by means of strain gauge force transducers.: "Pyloric opening and closure evaluated by means of strain gauge force transducers."J. Smooth Muscle Res. 34. 13-22 (1998)

通过应变仪力传感器评估幽门的打开和关闭。：“通过应变仪力传感器评估幽门的打开和关闭。”J。