Study on Reconstruction of the Hepatic Artery and Superior Mesenteric Artery Using a Cryopreserved Graft

冷冻保存移植物重建肝动脉和肠系膜上动脉的研究

• 批准号: 10671194
• 负责人: SATO Motomichi
• 金额: $ 1.98万
• 依托单位: EHIME UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671194/
• 关键词: Hepatic ischemia; arterial reconstruction; cryopreservation; neutrophil; reperfusion injury; aorta; peritonitis; 肝動脈; 血管移植; 感染

Reconstruction of the hepatic artery and superior mesenteric artery is challenging in hepatobiliary surgery. The purpose of this study is to evaluate the feasibility of cryopreserved arterial grafting and to eliminate intraoperative hepatic ischemia during these procedures.1. The aorta of Wister rats was cryopreserved with a program-freezer. The preserved aorta was transplanted to a recipient rat using a microsurgical technique under the condition of purulent peritonitis. Histologically, the grafted aorta showed no infection and a normal process after cryopreserved arterial grafting, including temporary endothelial cell loss and intimal hyperplasia.2. Reconstruction of the hepatic artery and superior mesenteric artery of pigs was difficult because of thick lymphatic channels.3. Rats receiving 90-min hepatic ischemia were divided into group C (control), group U treated with Urge-8, an antineutrophil antibody andgroup F treated with FK506, an immunosuppressor. Liver enzymes, pH, interleukin-8 (IL-8), and arterial ketone body ratio (AKBR) were measured 1 hour and 1 week after hepatic reperfusion. Survival rates were compared. Histologic damages of the liver were studied and the number of hepatic neutrophil infiltration was counted using ASD staining. One week-survival rate was tended to be lower in group C (12/20) than in groups U (16/20) and F (15/19). One hour after reperfusion of the liver, levels of GOT, GPT, LDH, and IL-8, hepatic neutrophil counts, and degree of hepatic damages were higher in group C than in groups U and F. Most of these changes subsided 1 week after grafting.4. Although hepatic tissue PO2 levels showed the presence of hepatic ischemia, it did not correlate to the degree of hepatic ischemic injury.In conclusion, this study indicated the safety of cryopreserved arterial grafting in the presence of peritnitis and efficacy of netrophil suppression therapy for hepatic ischemia

肝动脉和肠系膜上动脉的重建在肝胆外科中具有挑战性。本研究的目的是评估深低温保存动脉移植的可行性，并在这些过程中消除术中肝缺血。用程序冷冻机对Wistar大鼠的主动脉进行冷冻保存。在化脓性腹膜炎条件下，用显微外科技术将保存的主动脉移植给受体大鼠。结果：1.组织学上，移植动脉未见感染，移植动脉移植过程正常，包括一过性内皮细胞丢失和内膜增生。猪肝动脉和肠系膜上动脉因淋巴管粗大，重建困难。大鼠肝脏缺血90min后，随机分为C组(对照组)、U组(抗中性粒细胞抗体)和F组(免疫抑制剂FK506)。分别于再灌流后1h和1周检测肝酶、pH、白介素8(IL-8)和动脉血酮体比(AKBR)。比较两组的存活率。观察肝脏组织学损害，并用ASD染色计数肝脏中性粒细胞的数量。C组1周存活率(12/20)明显低于U组(16/20)和F组(15/19)。肝脏再灌流1h后，C组GOT、GPT、LDH、IL-8水平、肝脏中性粒细胞计数及肝组织损伤程度均高于U组和F组，移植后1周上述变化基本消失。尽管肝组织PO2水平显示了肝脏缺血的存在，但它与肝脏缺血损伤的程度无关。总之，本研究表明，在存在腹膜炎的情况下，深低温保存的动脉移植是安全的，网织红细胞抑制疗法对肝脏缺血是有效的

项目成果

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Motomichi Sato等人：“猪肝移植的临时主动脉分流技术”移植论文集。

Han SL: "The rapeutic efficacy of an antineutrophil monoclonal antibody, Urge-8, against acute necrotizing pancreatitis in rats"Surgery. 119. 585-591 (1996)

Han SL：“抗中性粒细胞单克隆抗体 Urge-8 对大鼠急性坏死性胰腺炎的治疗效果” 外科。

Chen YX: "Protecter effect of antineutrophil antibody,Urge-8,on liver ischemia-neperfuion infung."Transplantation Proceedings. 印刷中. (2000)

陈YX：“抗中性粒细胞抗体Urge-8对肝脏缺血-灌注的保护作用。”移植论文集（2000）。

Han SL: "Therapeutic efficacy of an antineutrophil monoclonal antibody Urge-8,agaimst acute necrotizing pancreatitis."Surgery. 119. 585-591 (1996)

Han SL：“抗中性粒细胞单克隆抗体Urge-8对急性坏死性胰腺炎的治疗效果。”外科。